Upregulation of mesencephalic astrocyte-derived neurotrophic factor in glial cells is associated with ischemia-induced glial activation
| Autor: | Facai Wang, Yuxian Shen, Shengyun Fang, Yujun Shen, Da-Qin Cha, Lijie Feng, Aimin Sun, Haiping Wang, Yunhong Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Immunology Ciliary neurotrophic factor lcsh:RC346-429 Rats Sprague-Dawley Cellular and Molecular Neuroscience Pregnancy Neurotrophic factors Internal medicine Glial Fibrillary Acidic Protein medicine Glial cell line-derived neurotrophic factor Animals Nerve Growth Factors Enzyme Inhibitors Glial activation Hypoxia Cells Cultured lcsh:Neurology. Diseases of the nervous system Neurons biology Glial fibrillary acidic protein Microglia Research General Neuroscience Brain Infarction Middle Cerebral Artery Cerebral ischemia Embryo Mammalian Cyclic Nucleotide Phosphodiesterases Type 3 Rats Cell biology Disease Models Animal Glucose medicine.anatomical_structure Endocrinology Nerve growth factor Gene Expression Regulation Neurology Mesencephalic astrocyte-derived neurotrophic factor biology.protein Endoplasmic reticulum stress Neuroglia Female Propidium Astrocyte |
| Zdroj: | Journal of Neuroinflammation, Vol 9, Iss 1, p 254 (2012) Journal of Neuroinflammation |
| ISSN: | 1742-2094 |
| Popis: | Background Mesencephalic astrocyte-derived neurotrophic factor (MANF), a 20 kDa secreted protein, was originally derived from a rat mesencephalic type-1 astrocyte cell line. MANF belongs to a novel evolutionally conserved family of neurotrophic factors along with conserved dopamine neurotrophic factor. In recent years, ever-increasing evidence has shown that both of them play a remarkable protective role against various injuries to neurons in vivo or in vitro. However, the characteristics of MANF expression in the different types of glial cells, especially in astrocytes, remain unclear. Methods The model of focal cerebral ischemia was induced by rat middle cerebral artery occlusion. Double-labeled immunofluorescent staining was used to identify the types of neural cells expressing MANF. Primarily cultured glial cells were used to detect the response of glial cells to endoplasmic reticulum stress stimulation. Propidium iodide staining was used to determine dead cells. Reverse transcription PCR and western blotting were used to detect the levels of mRNA and proteins. Results We found that MANF was predominantly expressed in neurons in both normal and ischemic cortex. Despite its name, MANF was poorly expressed in glial cells, including astrocytes, in normal brain tissue. However, the expression of MANF was upregulated in the glial cells under focal cerebral ischemia, including the astrocytes. This expression was also induced by several endoplasmic reticulum stress inducers and nutrient deprivation in cultured primary glial cells. The most interesting phenomenon observed in this study was the pattern of MANF expression in the microglia. The expression of MANF was closely associated with the morphology and state of microglia, accompanied by the upregulation of BIP/Grp78. Conclusions These results indicate that MANF expression was upregulated in the activated glial cells, which may contribute to the mechanism of ischemia-induced neural injury. |
| Databáze: | OpenAIRE |
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