Determination of expression profile of p53 gene in different grades of breast cancer tissues by real time PCR
| Autor: | Muhammad Riaz, Tariq Nadeem, Kanwal Ashiq, Ali Akbar, Kausar Malik, Rais Ahmed, Beenish Zahid, Sana Ashiq, Asma Irshad, Haleema Sadia, Munir Ahmad Bhinder |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Oncology
medicine.medical_specialty Tumor suppressor gene 030231 tropical medicine Down-Regulation Breast Neoplasms Real-Time Polymerase Chain Reaction Genome 03 medical and health sciences Breast cancer 0302 clinical medicine Internal medicine Complementary DNA Gene expression Humans Medicine Pakistan Epigenetics Gene Mastectomy business.industry p53 gene Articles General Medicine Middle Aged Genes p53 medicine.disease Up-Regulation Real-time polymerase chain reaction Punjab Female Neoplasm Grading Tumor Suppressor Protein p53 real-time PCR business |
| Zdroj: | African Health Sciences |
| ISSN: | 1680-6905 |
| Popis: | Background: Pakistan has a high incidence of breast cancer in Asia, where annually 16,232 deaths are reported. There are many exogenous and endogenous risk factors that affect the tumor suppressor genes and oncogenes. The p53 gene is a tumor suppressor gene and it has a role to protect the whole genome from external and internal stresses, which causes damages to the genome. Objective: The aim of the current study was to investigate the p53 gene expression by using the real-time PCR technique in different grades of breast cancer as compared to the normal tissue. Methods: Fresh Modified Radical Mastectomy (MRM) samples (grade1-grade3) were collected from different hospitals of the Lahore. The project was approved by an ethical review committee of Jinnah Hospital, Lahore. And before sampling an informed consent was obtained from patients and clinicians. RNA from fresh biopsies was extracted by Qiagen extraction kit and cDNA was formed. Real time PCR performed by using SYBR green master mix (ABI) and the data was evaluated by using Livak method. Statistical analysis was done by using Microsoft Excel. Results: There was an abnormal gene expression of p53 in all grades of the breast tumors. Non-significant (p>0.05) differ- ence of down and up regulation of p53 in different grades of breast tumor was found. However, as a whole up-regulation was more than down-regulation with significant difference (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|