Optimization of soybean dna extraction under different storage and development periods
| Autor: | Adriana de Andrade Figueiró, Ana Carolina Oliveira Mesquita, Karla Rodrigues Couto, Marília de Freitas Oliveira, Fernando Cezar Juliatti |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Developmental stage
Glycine max QH301-705.5 Agricultural Sciences Extraction (chemistry) Dna concentration glycine max Agriculture CTAB Biology DNA extraction Molecular degradation chemistry.chemical_compound Horticulture chemistry Botany Tukey's range test molecular biology ctab Biology (General) General Agricultural and Biological Sciences Molecular Biology Completely randomized design DNA |
| Zdroj: | Bioscience Journal ; Vol. 31 No. 4 (2015): July/Aug.; 1102-1106 Bioscience Journal ; v. 31 n. 4 (2015): July/Aug.; 1102-1106 Bioscience journal Universidade Federal de Uberlândia (UFU) instacron:UFU Bioscience Journal, Vol 31, Iss 4 (2015) |
| ISSN: | 1981-3163 |
| Popis: | DNA extraction of plants with high quality is very important to researches in molecular biology. Several extraction protocols have been used to obtain soybean DNA; however, there is a lack of papers about extraction protocols optimization and the best developmental stage of the plant to collect them. Therefore, the main purpose of the study was to extract high quantity and quality of DNA from fresh or frozen soybean samples, using different protocols. Moreover, we analyzed the best developmental stage of the plant to do the extraction. Fresh leaves or leaves kept for two years in the ultra-freezer were submitted to the DNA extraction protocols: Haberer et al., 1996 (modified); second modification from Haberer et al., 1996; Murray & Thompson, 1980 (modified) e Doyle & Doyle, 1990 (modified). Modified protocol of Doyle & Doyle was used to value the best stage to collect the leaves to do the DNA extraction. The samples were collected in the stages of development VC, V1, V2, V3, V4 and R5. The experiments were conducted in completely randomized design with 10 samples per treatment. The data underwent variance analysis and the averages were compared by the Tukey test (p |
| Databáze: | OpenAIRE |
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