Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus
| Autor: | Nathan Krishnan, Ravindran Kumaran, Sarah M. Dermody, Andrew Walls, Earl H. Harley |
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| Rok vydání: | 2015 |
| Předmět: |
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medicine.medical_specialty Obsessive-Compulsive Disorder Exacerbation Streptococcus pyogenes medicine.medical_treatment Antigens CD19 Palatine Tonsil Gastroenterology Group A Autoimmune Diseases 03 medical and health sciences 0302 clinical medicine PANDAS Internal medicine Streptococcal Infections B-Cell Activating Factor medicine Humans Prospective Studies Prospective cohort study Child B-Lymphocytes Sleep Apnea Obstructive business.industry General Medicine medicine.disease Pathophysiology Tonsillectomy Obstructive sleep apnea medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Tonsil Child Preschool Pediatrics Perinatology and Child Health Immunology Female business 030217 neurology & neurosurgery B-Cell Activation Factor Receptor |
| Zdroj: | International journal of pediatric otorhinolaryngology. 80 |
| ISSN: | 1872-8464 |
| Popis: | Objective To determine if Pediatric Autoimmune Neuropsychiatric Disorder Associated with Streptococcus (PANDAS) patients demonstrate a significantly different number of B-Cells or markers of activity when compared to recurrent Group A Streptococcus or Obstructive Sleep Apnea patients. Study design Prospective Cohort Study. Study setting Academic University Hospital. Methods Tonsil tissue was collected from twenty-two patients in the operating room and organized into three groups. Ten clinically diagnosed PANDAS, six Group A Streptococcus and six Obstructive Sleep Apnea patients were included in this study. Each tissue sample was extracted with MSD Tris Lysis Buffer and protein lysates were analyzed for CD 19, B-Cell Activating Factor and B-Cell Activating Receptor by western blot methods. Results Based on ANOVA analysis, there was no significant difference in the expression of B-Cell Activating Factor, B-Cell Activating Receptor or CD 19 when comparing the three study groups by western blot analysis methods. Conclusions In this prospective cohort study, it appears that PANDAS patients do not demonstrate increased number of B-Cells, expression of B-Cell Activating Factor or B-Cell Activating Receptor when compared to Group A Streptococcus or Obstructive Sleep Apnea cohorts. As a result, further evaluation of the cell-mediated immune system is warranted to provide further insight into the pathophysiology of PANDAS. In addition, we must investigate if PANDAS patients only demonstrate increased B-Cell number or activity when undergoing an acute Tic/OCD exacerbation. |
| Databáze: | OpenAIRE |
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