Plasma kinetics of an LDL-like non-protein nanoemulsion and transfer of lipids to high-density lipoprotein (HDL) in patients with rheumatoid arthritis
| Autor: | Fernanda Santos Pozzi, Raul C. Maranhão, C.G. Vinagre, Eduardo Ferreira Borba, Ieda Maria Magalhães Laurindo, Eloisa Bonfa, Lissiane Karine Noronha Guedes |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Apolipoprotein E
Adult Male medicine.medical_specialty Apolipoprotein B Endocrinology Diabetes and Metabolism Tritium Arthritis Rheumatoid chemistry.chemical_compound High-density lipoprotein Internal medicine Internal Medicine medicine Humans Carbon Radioisotopes Triglycerides Aged Apolipoproteins B Nutrition and Dietetics biology business.industry Cholesterol Cholesterol HDL Metabolism Cholesterol LDL Middle Aged medicine.disease Lipids Nanostructures Lipoproteins LDL Kinetics Endocrinology Treatment Outcome chemistry Rheumatoid arthritis Injections Intravenous biology.protein lipids (amino acids peptides and proteins) Apolipoprotein A1 Emulsions Female Cholesterol Esters Cardiology and Cardiovascular Medicine business Lipoproteins HDL Lipoprotein |
| Zdroj: | Journal of clinical lipidology. 9(1) |
| ISSN: | 1933-2874 |
| Popis: | Rheumatoid arthritis (RA) is a systemic inflammatory disease associated with cardiovascular risk, but with normal plasma lipids.The aim was to investigate low-density lipoprotein (LDL) and high-density lipoprotein (HDL) metabolism in RA patients using radioactive nanoemulsions resembling an LDL lipid structure (LDE) as metabolic probes.Thirty patients with RA, 16 in remission and 14 in high activity, and 30 healthy controls were studied. LDE labeled with (14)C-cholesteryl ester ((14)C-CE) and (3)H-unesterified cholesterol ((3)H-UC) was intravenously injected followed by 24-hour plasma sampling. Fractional clearance rates (FCR, h(-1)) were calculated by compartmental analysis. Lipid transfers to HDL were assayed by incubating plasma samples with a donor nanoemulsion labeled with radioactive lipids; % lipids transferred to HDL were quantified after chemical precipitation.LDL cholesterol, triglycerides, unesterified cholesterol, and oxidized LDL were equal in RA and controls, and HDL cholesterol was even higher in RA. Compared with controls, apolipoprotein B was lower, apolipoprotein A1 was equal, and apolipoprotein E was higher in RA. Decay curves of LDE labels were faster in RA patients than in controls ((14)C-CE: 0.072 ± 0.066 and 0.038 ± 0.027, P = .0115; (3)H-UC: 0.066 ± 0.042 and 0.035 ± 0.039; P .0044). FCRs were equal in 2 RA subgroups. Transfer of UC, triglycerides, and phospholipids to HDL was equal between RA and controls, but CE transfer was lower in RA. HDL size was smaller in RA patients than in controls (8.5 ± 0.5 nm; 9.2 ± 0.8 nm, P .0001).RA patients were more efficient in removing atherogenic LDL from plasma, as indicated by higher CE and UC FCR, with in lower apolipoprotein B. This was unexpected because of the higher cardiovascular risk in RA. |
| Databáze: | OpenAIRE |
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