Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store depletion

Autor: Katsuhiko Mikoshiba, Sophia Smida-Rezgui, Michel Ronjat, Clara Franzini-Armstrong, Francesco Zorzato, Luca Moccagatta, Cristiano Grasso, Susan Treves, Adam G. Schrum, Sylvie Ducreux, Christophe Arnoult, Thierry Girard, Michael X. Zhu
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Cytoplasm
Time Factors
Muscle Proteins
Receptors
Cytoplasmic and Nuclear
N-type calcium channel
Endoplasmic Reticulum
Calcium in biology
Mixed Function Oxygenases
Calcium-binding protein
Junctate
Inositol 1
4
5-Trisphosphate Receptors
Research Articles
calcium store
Ryanodine receptor
calcium homeostasis
IP3R
calcium entry channel
calcium binding protein
ER
Flow Cytometry
Cell biology
Electrophysiology
Sarcoplasmic Reticulum
COS Cells
Electrophoresis
Polyacrylamide Gel
RNA Interference
SOCE
Subcellular Fractions
Calcium pump
Green Fluorescent Proteins
Immunoblotting
chemistry.chemical_element
Biology
Calcium
Transfection
Article
Cell Line
Calcium influx
Cations
Animals
Humans
IP3Receptor
RNA
Double-Stranded
Calcium-Binding Proteins
Cell Membrane
T-type calcium channel
Membrane Proteins
Cell Biology
Precipitin Tests
Protein Structure
Tertiary
Calcium ATPase
Luminescent Proteins
Microscopy
Electron
chemistry
Type C Phospholipases
Calcium Channels
Peptides
Zdroj: The Journal of Cell Biology
ISSN: 1540-8140
0021-9525
Popis: In many cell types agonist-receptor activation leads to a rapid and transient release of Ca2+ from intracellular stores via activation of inositol 1,4,5 trisphosphate (InsP3) receptors (InsP3Rs). Stimulated cells activate store- or receptor-operated calcium channels localized in the plasma membrane, allowing entry of extracellular calcium into the cytoplasm, and thus replenishment of intracellular calcium stores. Calcium entry must be finely regulated in order to prevent an excessive intracellular calcium increase. Junctate, an integral calcium binding protein of endo(sarco)plasmic reticulum membrane, (a) induces and/or stabilizes peripheral couplings between the ER and the plasma membrane, and (b) forms a supramolecular complex with the InsP3R and the canonical transient receptor potential protein (TRPC) 3 calcium entry channel. The full-length protein modulates both agonist-induced and store depletion–induced calcium entry, whereas its NH2 terminus affects receptor-activated calcium entry. RNA interference to deplete cells of endogenous junctate, knocked down both agonist-activated calcium release from intracellular stores and calcium entry via TRPC3. These results demonstrate that junctate is a new protein involved in calcium homeostasis in eukaryotic cells.
Databáze: OpenAIRE
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