Genomic identification and expression analysis of nuclear pore proteins in Malus domestica
| Autor: | Libo Xing, Chenguang Zhang, Wei Zhang, Mingyu Han, Jiayan Liang, Peng Jia, Hua Zhou, Xu Zhang, Xiaolin Ren, Wenchun Ma, Na An |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Malus Protein domain Arabidopsis lcsh:Medicine 01 natural sciences Article 03 medical and health sciences Databases Genetic Developmental biology Genetics Arabidopsis thaliana Nuclear pore lcsh:Science Gene Phylogeny Plant Proteins Multidisciplinary biology Phylogenetic tree Gene Expression Profiling fungi lcsh:R Intron food and beverages biology.organism_classification Protein Structure Tertiary Nuclear Pore Complex Proteins 030104 developmental biology Nuclear Pore lcsh:Q Nucleoporin Plant sciences Genome Plant 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-74171-0 |
| Popis: | The nuclear pore complex (NPC), comprised of individual nucleoporin (Nup) proteins, controls nucleo-cytoplasmic transport of RNA and protein, and is important for regulating plant growth and development. However, there are no reports on this complex in fruit tree species. In this study, we identified 38 apple Nups and named them based on the known Arabidopsis thaliana homologs. We also completed bioinformatics analyses of the intron and exon structural data for apple Nups. The proteins encoded by the apple Nups lacked a universally conserved domain. Moreover, a phylogenetic analysis separated the apple and A. thaliana Nups into three groups. The phylogenetic tree indicated that MdNup54 and MdNup62 are most closely related to genes in other Rosaceae species. To characterize the 38 candidate Malus domestica Nups, we measured their stage-specific expression levels. Our tests revealed these proteins were differentially expressed among diverse tissues. We analyzed the expression levels of seven apple Nups in response to an indole-3-acetic acid (IAA) treatment. The phytohormone treatment significantly inhibited apple flowering. A qRT-PCR analysis proved that an IAA treatment significantly inhibited the expression of these seven genes. A preliminary study regarding two members of the Nup62 subcomplex, MdNup54 and MdNup62, confirmed these two proteins can interact with each other. A yeast two-hybrid assay verified that MdNup54 can interact with MdKNAT4 and MdKNAT6. On the basis of the study results, we identified apple NPC and predicted its structure and function. The data generated in this investigation provide important reference material for follow-up research. |
| Databáze: | OpenAIRE |
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