| Autor: |
De Kleijn, Kim M. A., Zuure, Wieteke A., Straasheijm, Kirsten R., Martens, Marijn B., Avramut, M. Cristina, Koning, Roman I., Martens, Gerard J. M. |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22617478.v1 |
| Popis: |
Additional file 4: Fig. S4. Complete omission of SMAD inhibition (absence of dorsomorphin and SB43152 in the culture medium) results in mitigated growth of neuroectoderm-derived neurons and astrocytes. (A) Representative images of whole-spheroid signal scans of the neuronal marker MAP2, the astrocyte marker GFAP and the oligodendrocyte marker O1 in day-17 (d17), d33 and d73 human cortical spheroids (hCSs) (batch-11) grown with dorsomorphin and SB-431542 (+ SMAD inhibition) or without dorsomorphin and SB-431542 (- SMAD inhibition) in the culture medium. (B) Quantifications of the mean MAP2-, GFAP- and O1-signal intensities per field in d17, d33 and d73 hCSs (batch-11) grown with or without SMAD inhibition. One-way ANOVA per time point for -SMAD vs +SMAD: * p < 0.05. Each data point represents the level of mRNA expression in one spheroid. Error bars represent the standard error of the mean. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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