Lentiviral Vectors Pseudotyped with Envelope Glycoproteins Derived from Gibbon Ape Leukemia Virus and Murine Leukemia Virus 10A1
| Autor: | Wolfgang Uckert, Jörn Stitz, Isabel Schmitt, Christian J. Buchholz, Klaus Cichutek, U. Bloemer, Martin Engelstädter |
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| Jazyk: | angličtina |
| Předmět: |
Genetic enhancement
Recombinant Fusion Proteins viruses Genetic Vectors Molecular Sequence Data Clone (cell biology) 10A1 Env Viral Plaque Assay Biology Transfection Viral vector Cell Line Mice Dogs Virology Murine leukemia virus Animals Humans Vector (molecular biology) Amino Acid Sequence chemistry.chemical_classification AIDS Vaccines Vaccines Synthetic lentiviral vector GaLV Env Gene Products env pseudotype vector biology.organism_classification Flow Cytometry Leukemia Virus Murine chemistry Vesicular stomatitis virus Leukemia Virus Gibbon Ape HIV-1 Glycoprotein |
| Zdroj: | Virology. (1):16-20 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2000.0394 |
| Popis: | Lentiviral vectors pseudotyped with the envelope glycoproteins (Env) of amphotropic murine leukemia virus (MLV) and the G protein of vesicular stomatitis virus (VSV-G) have been successfully used in recent preclinical gene therapy studies. We report here the generation of infectious HIV-1-derived vector particles pseudotyped with the Env of the molecular clone 10A1 of MLV and with chimeric envelope glycoprotein variants derived from gibbon ape leukemia virus (GaLV) and MLV. Formation of infectious HIV-1 (GaLV) pseudotype vectors was only possible with the substitution of the cytoplasmic tail of GaLV Env with that of MLV. The lentiviral vectors exhibited a host cell range identical with that of MLV(GaLV) and MLV(10A1) vectors, which are known to enter cells either via the GaLV-receptor Glvr-1 (Pit-1) or via the amphotropic receptor Ram-1 (Pit-2) in addition to Glvr-1, respectively. Thus, HIV-1(GaLV) and HIV-1(10A1) pseudotype vectors may be useful for efficient gene transfer into a variety of human tissues like primary human hematopoietic cells. |
| Databáze: | OpenAIRE |
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