Determination of Dihydroergosine in Sorghum Ergot Using an Immunoassay
| Autor: | Christopher J. Moore, J.B. Molloy, Sally-Ann Murray, Anthea G. Bruyeres, B. J. Blaney |
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| Rok vydání: | 2003 |
| Předmět: |
Animal feed
Enzyme-Linked Immunosorbent Assay Food Contamination medicine.disease_cause Sensitivity and Specificity Claviceps chemistry.chemical_compound medicine Bovine serum albumin Mycotoxin Chromatography High Pressure Liquid Chromatography biology medicine.diagnostic_test Toxin Alkaloid General Chemistry Animal Feed Ergotamines Biochemistry chemistry Polyclonal antibodies Immunoassay Ergotamine biology.protein Edible Grain General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 51:3916-3919 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf0212284 |
| Popis: | Dihydroergosine (DHES) is the principal toxic alkaloid produced by sorghum ergot (Claviceps africana). It has recently been shown that DHES levels as low as 1 mg/kg in animal feed can cause significant production losses. Quantitative immunoassays for detecting the related rye ergot alkaloid, ergotamine, are described in the literature, but those assays are relatively insensitive for DHES. This paper describes competitive enzyme-linked immunosorbent assays (ELISA) for measuring the DHES concentration in grains and mixed animal feed. The assays were developed using a DHES specific mouse monoclonal antibody and rabbit polyclonal antibodies raised against DHES conjugated to bovine serum albumin. Recoveries of between 77 and 103% were obtained from spiked grain using a simple, one step extraction with 70% methanol. Both the monoclonal and the polyclonal assays are capable of detecting DHES concentrations above 0.01 mg/kg, but quantification is most reliable at concentrations of 0.1 mg/kg or higher. |
| Databáze: | OpenAIRE |
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