In vitro induction of tetraploidy in mulberry (Morus alba L.)
| Autor: | B. N. Roy, S. M. H. Qadri, S. P. Chakraborti, K. Vijayan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
1-Naphthaleneacetic acid biology Apical dominance Plant Science General Medicine Moraceae biology.organism_classification Horticulture chemistry.chemical_compound Tissue culture Murashige and Skoog medium chemistry Auxin Botany Colchicine Agronomy and Crop Science Explant culture |
| Zdroj: | Plant cell reports. 17(10) |
| ISSN: | 1432-203X |
| Popis: | A high frequency of tetraploidy was induced in mulberry (Morus alba L.) through apical bud treatment under in vitro conditions. Apical buds from in vitro-grown plants were treated with three different concentrations (0.05, 0.1 and 0.2%) of colchicine in MS medium for 24 h. Tetraploidy at a frequency of 39.4±4.8% was obtained using 0.1% colchicine, whereas the frequency of tetraploidy was significantly reduced to 16.7±2.3% when 0.2% colchicine was used. Morphological, histological and cytological evidence indicated a phenotypic and genomic similarity of in vitro- with ex vitro-induced tetraploids. Rooting of tetraploids was on basal medium containing 2.6 μm NAA. The recovery of tetraploids was 80.8% more efficient using the in vitro method instead of the ex vitro method. The use of the same colchicine medium for up to 4 weeks with additional explants was found to be equally effective for the induction of tetraploidy. |
| Databáze: | OpenAIRE |
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