Determination of arsenic chemical species in rice grains using high-performance liquid chromatography coupled to hydride generator with atomic fluorescence detector (HPLC-HG-AFS)
| Autor: | José Marrugo-Negrete, Germán Enamorado-Montes, Luis Ángel Ospina Herrera, Iván Urango-Cárdenas, Saudith Burgos-Núñez |
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| Rok vydání: | 2020 |
| Předmět: |
Analytical validation
Science Clinical Biochemistry chemistry.chemical_element (LOD) Limits of detection (LOQ) Limits of quantification 010501 environmental sciences 01 natural sciences High-performance liquid chromatography 03 medical and health sciences chemistry.chemical_compound HPLC-HG-AFS High-performace liquid chromatography coupled to hydride generator with atomic fluorescence detector arsenic species by HPLC-HG-AFS in rice grain LGS liquid gas separator Arsenic 030304 developmental biology 0105 earth and related environmental sciences Arsenite Detection limit 0303 health sciences Reproducibility Chromatography Chemistry As (III) arsenite Anion exchange chromatography Extraction (chemistry) Arsenate As (V) arsenate food and beverages Repeatability Cereal grain Method Article Medical Laboratory Technology MMA monomethylarsonic acid MAE microwave assisted extraction DMA dimethylarsinic acid |
| Zdroj: | MethodsX MethodsX, Vol 8, Iss, Pp 101281-(2021) |
| ISSN: | 2215-0161 |
| Popis: | The objective of this work is the validation and implementation of an analytical method for the determination of arsenic chemical species in rice grain samples using High-performance liquid chromatography coupled to a hydride generator with atomic fluorescence detector (HPLC-HG-AFS). The extraction protocol developed was based on HNO3 0.28 M (90 °C, 2 h), microwave-assisted. The results showed recovery percentages of arsenite (As (III)) (99–101%), arsenate (As (V)) (91–96%), dimethylarsinic acid (DMA) (92–102%) and monomethylarsonic acid (MMA) (94–97%). The precision of the method presented coefficients of variation lower than 7% and 8% for repeatability and reproducibility respectively. The detection limits were 2.5, 3.75, 7.5 and 4.0 µg kg−1 for As (III), As (V), DMA and MMA respectively. The proposed methodology is reliable for the quantification of As species, because they are conserved during the extraction.•The extraction protocol developed was based on Microwave-assisted acid extraction.•This methodology offers good sensitivity, precision, accuracy, detection and quantification limits.•It was successfully applied to determination of arsenic chemical species in rice grains. Graphical abstract Image, graphical abstract |
| Databáze: | OpenAIRE |
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