Interaction of Catechu Dye with DNA: Spectroscopic and In Silico Approach
Autor: | R. Priya, Ramamoorthy Siva, Hridya Hemachandran, George Priya Doss, Amrita Anantharaman |
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Rok vydání: | 2016 |
Předmět: |
Circular dichroism
Analytical chemistry 02 engineering and technology Catechu 010402 general chemistry 01 natural sciences Biochemistry Catechin Fluorescence spectroscopy chemistry.chemical_compound Genetics Molecule Fourier transform infrared spectroscopy Acacia catechu biology Chemistry Spectrum Analysis DNA General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Fluorescence 0104 chemical sciences Molecular Docking Simulation Molecular Medicine 0210 nano-technology Ethidium bromide Hydrophobic and Hydrophilic Interactions Nuclear chemistry |
Zdroj: | Nucleosides, Nucleotides and Nucleic Acids. 35:195-210 |
ISSN: | 1532-2335 1525-7770 |
Popis: | Catechin, a yellow colored molecule obtained from the wood of Acacia catechu was analyzed for its interaction with synthetic DNA duplexes using spectroscopic analysis. UV-Visible spectroscopic analysis revealed the non-intercalative binding mode. Fourier Transform Infrared spectroscopy (FTIR) analysis expose chemical shift indicated by various vibrational stretches and an increase in the intensity of base stacking was observed by Circular Dichroism (CD), respectively. This inference was further confirmed through nuclear staining technique and also in electrophoretic technique; the dye quenches the fluorescent intensity of ethidium bromide. The result of fluorescence spectroscopy was in concordance with the electrophoretic technique. In addition, the spectroscopic results were in accordance with the molecular docking studies of specific catechin compound from the catechu dye with CT-DNA. This kind of site specificity is a gain in the medicinal field as the drug can be DNA targeted for cancer therapeutics. The present work reveals that catechu dye has a noteworthy application in the field of medical bioscience. |
Databáze: | OpenAIRE |
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