Protection of teprenone against hypoxia and reoxygenation stress in stomach and intestine of Lateolabrax maculatus
| Autor: | Yafei Duan, Jiasong Zhang, Hongbiao Dong, Wenhao Wang, Mujiao Xie, Hua Li, Liu Qingsong, Yongxu Sun, AiJun Zhan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Physiology Apoptosis Aquatic Science Biology medicine.disease_cause Biochemistry Immunity Stress Physiological Internal medicine medicine Animals HSP70 Heat-Shock Proteins Hypoxia Gastrointestinal tract Stomach General Medicine Hypoxia (medical) Hsp70 Perciformes Intestines medicine.anatomical_structure Endocrinology Teprenone medicine.symptom Diterpenes Oxidative stress medicine.drug |
| Zdroj: | Fish physiology and biochemistry. 46(2) |
| ISSN: | 1573-5168 |
| Popis: | Teprenone (geranylgeranylacetone) is one kind of safe and effective agent in gastrointestinal mucosa, which have been widely used in human and veterinary, but rarely used in aquaculture animals. In this study, Lateolabrax maculatus, an important economic fish species in southern China, was taken as the object of study to investigate the protective effect of teprenone on intestinal stress. The present study was designed to investigate the potential mechanism underlying the protection offered by teprenone to protect the gastrointestinal tract against hypoxia and reoxygenation injury of L. maculatus. (a) For oxidative stress parameters, SOD, CAT, and T-AOC in control group were higher than those in teprenone group. MDA content was significantly higher than that in teprenone group at N and 12h time points in intestine (P < 0.05), and at 12, 24, and 48 h time points in stomach. (b) For immune-associated proteins, LZM activity in the control group was lower than that in the teprenone group, and the difference between the two groups in stomach and intestine was significant at 12.48 h and 6.48 h time points, respectively (P < 0.05). Compared with time point N, the content of HSP70 in the control group increased at 0 h in intestine. At 0–48 h, intestine HSP70 content in the control group showed a gradually decreasing trend, which was higher than that in the teprenone group. (c) For apoptosis-related factors, the activity of Cyt-C, caspase9, and caspase3 increased first and then decreased in both groups. The content of Cyt-C in the control group was significantly higher than that in the teprenone group at N-3.6 h, and 3.48 h time points in stomach and intestine, respectively (P |
| Databáze: | OpenAIRE |
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