A method for the purification of nicotinamide mononucleotide

Autor:	G.W.E. Plaut, Katharine Armstrong Plaut
Rok vydání:	1954
Předmět:	Pyrophosphatase Aqueous solution Chromatography Elution Nucleotides Biophysics Isoamyl alcohol Biochemistry Chloride chemistry.chemical_compound chemistry Acetone medicine Humans Molecular Biology Fluoride Nicotinamide Mononucleotide medicine.drug Nicotinamide mononucleotide
Zdroj:	Archives of biochemistry and biophysics. 48(1)
ISSN:	0003-9861
Popis:	Purified nicotinamide mononucleotide (NMN) has been prepared by incubation of diphosphopyridine nucleotide (DPN) with potato pyrophosphatase in a nonphosphate buffer and in the presence of fluoride. The NMN formed is adsorbed on charcoal followed by elution with aqueous isoamyl alcohol. This eluate is passed through Dowex 1 chloride, and NMN is washed out of the anion exchanger with water, DPN and adenylic acid being retained by the resin. NMN is precipitated from a slightly acidic solution with acetone.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b04ee68c970584e689d083d676fadcf https://pubmed.ncbi.nlm.nih.gov/13125588 Zobrazit plný text záznamu