A light-sensitive mutation in Arabidopsis LEW3 reveals the important role of N-glycosylation in root growth and development
| Autor: | P. J. Casero, José María García-Mina, Roberto Baigorri, Juan Carlos del Pozo, Matthew Norris, Miguel A. Moreno-Risueno, Ilda Casimiro, Mercedes Pallero-Baena, Sara Navarro Neila, Concepción Manzano, Yiliang Ding, Lourdes Rubio, José A. Fernández, Javier Silva-Navas |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Glycosylation Positional cloning Physiology RNA Splicing Mutant Meristem ROS Arabidopsis Plant Science N-glycosylation Mannosyltransferases Plant Roots 03 medical and health sciences Botany Peroxidase biology Arabidopsis Proteins Basidiomycota beta-Glucosidase Intron Hydrogen-Ion Concentration biology.organism_classification Introns Cell biology Root development 030104 developmental biology Phenotype Peroxidases Seedlings Mutation (genetic algorithm) RNA splicing Mutation Synonymous substitution Reactive Oxygen Species Cell Division |
| Zdroj: | Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA INIA: Repositorio de Resultados de Investigación del INIA |
| ISSN: | 1460-2431 |
| Popis: | Plant roots have the potential capacity to grow almost indefinitely if meristematic and lateral branching is sustained. In a genetic screen we identified an Arabidopsis mutant showing limited root growth (lrg1) due to defects in cell division and elongation in the root meristem. Positional cloning determined that lrg1 affects an alpha-1,2-mannosyltransferase gene, LEW3, involved in protein N-glycosylation. The lrg1 mutation causes a synonymous substitution that alters the correct splicing of the fourth intron in LEW3, causing a mix of wild-type and truncated protein. LRG1 RNA missplicing in roots and short root phenotypes in lrg1 are light-intensity dependent. This mutation disrupts a GC-base pair in a three-base-pair stem with a four-nucleotide loop, which seems to be necessary for correct LEW3 RNA splicing. We found that the lrg1 short root phenotype correlates with high levels of reactive oxygen species and low pH in the apoplast. Proteomic analyses of N-glycosylated proteins identified GLU23/PYK10 and PRX34 as N-glycosylation targets of LRG1 activity. The lrg1 mutation reduces the positive interaction between Arabidopsis and Serendipita indica. A prx34 mutant showed a significant reduction in root growth, which is additive to lrg1. Taken together our work highlights the important role of N-glycosylation in root growth and development. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email journals.permissions@oup.com. |
| Databáze: | OpenAIRE |
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