Distribution of two distinct rotavirus B (RVB) strains in the north-central Bangladesh and evidence for reassortment event among human RVB revealed by whole genomic analysis
| Autor: | Nobumichi Kobayashi, Yashpal Singh Malik, Satoru Aida, Shyamal Kumar Paul, Ayako Sumi, Meiji Soe Aung, Noriko Urushibara, S Nahar, Souvik Ghosh, Mitsuyo Kawaguchiya, Muhammad Akram Hossain, Nazia Haque, Salma Ahmed |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Rotavirus 0301 basic medicine Microbiology (medical) Lineage (genetic) viruses Reassortment Rotavirus Infections Genome Viral Biology Microbiology Viral Proteins Young Adult 03 medical and health sciences Genetics Humans Molecular Biology Gene Ecology Evolution Behavior and Systematics Aged Bangladesh NSP1 North central Strain (biology) Genetic Variation RNA Sequence Analysis DNA Middle Aged Virology 030104 developmental biology Infectious Diseases Sequence Alignment Reassortant Viruses |
| Zdroj: | Infection, Genetics and Evolution. 47:77-86 |
| ISSN: | 1567-1348 |
| DOI: | 10.1016/j.meegid.2016.11.001 |
| Popis: | Human rotavirus B (RVB), a rare cause of diarrhea in several Asian countries, has been reported to be genetically highly conserved. However, 14 RVB strains with two distinct RNA electropherotypes E1 and E2 (11 and 3 strains, respectively) were detected in adult patients with diarrhea, in Mymensingh in the north-central Bangladesh in 2014. In this study, VP7 gene sequences of all the 14 strains and nearly full-length sequences of all the 11 RNA segments of four RVB (two strains each representing E1 and E2 types) were determined and analyzed phylogenetically. For all the gene segments, sequence identities among strains with the same RNA pattern were higher (99%-100%) than those between strains with different RNA patterns (94-98%). Although all the gene segments of RVB strains were grouped into Indian-Bangladeshi lineage, VP1-3, VP6, VP7, NSP1, NSP2 and NSP5 genes of strains with E1 and E2 types were assigned to distinct sublineages S1 and S2, respectively. E1-strains clustered with Bangladeshi RVB strains reported previously (e.g., Bang117), while E2-strains with those from India (e.g., NIV-1048101), Myanmar, and Nepal. In contrast, VP4, NSP3 and NSP4 genes of both E1 and E2 RVB strains were classified into sublineage S2. These findings indicated that two genetically distinct RVB strains were simultaneously circulating in Mymensingh, Bangladesh. RVB strains with E1 electropherotype were suggested to be reassortants acquiring three gene segments (VP4, NSP3 and NSP4 genes) from the foreign RVB in the genetic background of indigenous Bangladeshi RVB represented by the strain Bang117. |
| Databáze: | OpenAIRE |
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