High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
| Autor: | Yongyuth Yuthavong, H.Kyle Webster, Isra Ittarat |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Oxidoreductases Acting on CH-CH Group Donors
Primaquine Plasmodium falciparum Dihydroorotate Dehydrogenase Dihydroorotate Oxidase Antimalarials Enzyme activator parasitic diseases Gametocyte medicine Animals Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography biology Chemistry General Chemistry biology.organism_classification Enzyme assay Enzyme Activation Enzyme Biochemistry Aminoquinolines biology.protein Dihydroorotate dehydrogenase Oxidoreductases Naphthoquinones medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 582:57-64 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(92)80302-7 |
| Popis: | A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gametocyte stages of P. falciparum in culture with menoctone, primaquine or the primaquine derivative WR 238605 led to depression of the enzyme activity, although the drugs did not appear to inhibit the enzyme directly. |
| Databáze: | OpenAIRE |
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