Development and Validation of an ICP-MS Method and Its Application to Determine Multiple Trace Elements in Small Volumes of Whole Blood and Plasma
| Autor: | P. Nicholas Shaw, Jack C. Ng, E. M. Tanvir, Karen Whitfield |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Health Toxicology and Mutagenesis chemistry.chemical_element 010501 environmental sciences Toxicology 01 natural sciences Mass Spectrometry Analytical Chemistry Plasma Selenium Humans Environmental Chemistry Inductively coupled plasma mass spectrometry 0105 earth and related environmental sciences Whole blood Cadmium Chemical Health and Safety Chromatography Spectrum Analysis 010401 analytical chemistry Trace element Barium Trace Elements 0104 chemical sciences Zinc chemistry Thallium Vacutainer Copper |
| Zdroj: | Journal of Analytical Toxicology. 44:1036-1046 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/bkaa033 |
| Popis: | Essential and nonessential element concentrations in human blood provide important information on the nutritional status of individuals and can assist in the screening or diagnosis of certain disorders and their association with other causative factors. A simple and sensitive method, suitable for use with small sample volumes, for quantification of multiple trace element concentrations in whole blood and plasma has been developed using inductively coupled plasma-mass spectrometry. Method validation was performed using standard reference materials of whole blood and serum using varying sample treatments with nitric acid, water and hydrogen peroxide. The method was applied to quantify the trace element concentrations in whole blood and plasma samples (0.1 mL) from 50 adult blood donors in Queensland. The whole blood sample (5 mL) was collected in Vacutainer tubes with K2EDTA as anticoagulant. The developed method was able to quantify, in blood and plasma samples over a wide range of concentrations, several essential elements: cobalt, copper, zinc, iron, manganese and selenium; the nutritionally probably essential elements vanadium and strontium; and nonessential elements including lead, cadmium, arsenic, caesium, barium, thallium and uranium. Significant differences (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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