TetrODrive: An open-source microdrive for combined electrophysiology and optophysiology
| Autor: | Michael T. Lippert, Alisa Vlasenko, Frank W. Ohl, Marcel Brosch |
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| Rok vydání: | 2020 |
| Předmět: |
3d printed
Computer science Mean squared prediction error 0206 medical engineering Biomedical Engineering 02 engineering and technology Optogenetics 3d printer Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Animals Electrodes Tetrode (biology) business.industry Brain 020601 biomedical engineering Cell identity Electrodes Implanted Electrophysiological Phenomena Electrophysiology Ventral tegmental area Open source medicine.anatomical_structure business Microelectrodes 030217 neurology & neurosurgery Computer hardware Biomedical engineering |
| Popis: | Objective. In tetrode recordings, the cell types of the recorded units are difficult to determine based on electrophysiological characteristics alone. Optotagging, the use of optogenetic stimulation to precisely identify cells, is a method to overcome this challenge. However, recording from many different cells requires advancing electrodes and light sources slowly through the brain with a microdrive. Existing designs suffer from a number of drawbacks, such as limited stability and precision, high cost, complex assembly, or excessive size and weight. Approach. We designed TetrODrive as a microdrive that can be 3D printed on an inexpensive desktop resin printer, has minimal parts, assembly time, and cost. The microdrive can be assembled in 15 min and the price for all materials, including the 3D printer, is lower than a single commercial microdrive. To maximize recording stability, we mechanically decoupled the drive mechanism from the electrical and optical connectors. Main results. The developed microdrive is small and light enough (Significance. TetrODrive is a tiny, lightweight, and affordable microdrive for optophysiology in mice. Its open design, price, and built-in characteristics can significantly expand the use of microdrives in mice. |
| Databáze: | OpenAIRE |
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