Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities
| Autor: | Ying Guo, Kai Liu, Xuehai Yan, Jiajia Sun, Yuri Lvov, Raghuvara Yendluri |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Nanotube
Aqueous solution biology Composite number General Physics and Astronomy 02 engineering and technology engineering.material 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Halloysite 0104 chemical sciences Chitosan chemistry.chemical_compound Membrane chemistry Biochemistry Coating Chemical engineering biology.protein engineering Physical and Theoretical Chemistry Lipase 0210 nano-technology |
| Zdroj: | Physical chemistry chemical physics : PCCP. 19(1) |
| ISSN: | 1463-9084 |
| Popis: | We have developed a simple and effective strategy to prepare an enzymatic membrane by the admixing of a halloysite clay nanotube–lipase complex and a chitosan solution. The loading of 3 wt% lipase into the lumen of halloysite clay nanotubes was enhanced with an electrostatic interaction. 10 wt% of lipase–nanotubes were dispersed in an aqueous chitosan solution and cast as a coating. The enzymatic chitosan–halloysite membranes displayed increased pH, temperature stability and long-term usability for the catalytic decomposition of lipids: 90% of the initial activity was preserved at 80 °C, 70% of the functionality remained at the high pH of 9, and 85% of the activity was sustained after ten continuous cycles of use. The placement of the second antimicrobial enzyme–lysozyme at the outer surface of the clay nanotubes allowed for a synergistic increase in the activity of the composite antibacterial formulation. The lipase/lysozyme-co-immobilized clay nanotube–chitosan membranes are promising in anti-biofouling applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|