Investigation of laser-induced cell lysis using time-resolved imaging

Autor:	Arnold Guerra, Vasan Venugopalan, Kaustubh R. Rau, Alfred Vogel
Rok vydání:	2004
Předmět:	Materials science Lysis Physics and Astronomy (miscellaneous) Plasma formation Microbeam irradiation Plasma medicine.disease Laser law.invention Nuclear magnetic resonance law Cavitation medicine Biophysics Pressure wave propagation Cell damage
Zdroj:	Rau, KR; Guerra, A; Vogel, A; & Venugopalan, V. (2004). Investigation of laser-induced cell lysis using time-resolved imaging. Applied Physics Letters, 84(15), 2940-2942. doi: 10.1063/1.1705728. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/7ns8r70n
ISSN:	1077-3118 0003-6951
DOI:	10.1063/1.1705728
Popis:	Using time-resolved imaging, we investigated the lysis of confluent PtK2 cell cultures by pulsed laser microbeam irradiation. Images obtained at time delays of 0.5 ns to 50 μs demonstrate lysis to be mediated by laser-induced plasma formation resulting in pressure wave propagation and cavitation bubble formation. Image analysis enabled quantitative characterization of the pressure wave and cavitation bubble dynamics. The zone of cell damage exceeded the plasma size and serves to implicate cavitation bubble expansion as the primary agent of cell injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af0d8d130f90cf7549388635ab839f3 https://doi.org/10.1063/1.1705728 Zobrazit plný text záznamu