Transplantation of Normal Adipose Tissue Improves Blood Flow and Reduces Inflammation in High Fat Fed Mice With Hindlimb Ischemia
| Autor: | Liyuan Chen, Lin Wang, Yongjie Li, Liqun Wuang, Yaofang Liu, Ningbo Pang, Yulin Luo, Jing He, Liping Zhang, Ni Chen, Rong Li, Jianbo Wu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology Macrophage polarization Adipose tissue White adipose tissue 030204 cardiovascular system & hematology lcsh:Physiology blood perfusion Neovascularization 03 medical and health sciences 0302 clinical medicine Insulin resistance Physiology (medical) Internal medicine Brown adipose tissue medicine Glucose homeostasis lcsh:QP1-981 business.industry Correction medicine.disease adipose tissue Transplantation 030104 developmental biology medicine.anatomical_structure Endocrinology high-fat diet arteriogenesis inflammation medicine.symptom business |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 9 (2018) |
| ISSN: | 1664-042X |
| Popis: | Background: Fat deposition is associated with peripheral arterial disease. Adipose tissue has recently been implicated in vascular remodeling and angiogenic activity. We hypothesized that the transplantation of adipose tissues from normal mice improves blood flow perfusion and neovascularization in high-fat diet fed mice. Methods: After 14 weeks of high-fat diet (HFD)-fed mice, unilateral hind limb ischemia was performed. Subcutaneous white adipose tissue (WAT) and brown adipose tissue (BAT) fat pads were harvested from normal EGFP mice, and subcutaneously transplanted over the region of the adductor muscles of HFD mice. Blood flow was measured using Laser Doppler Scanner. Vascular density, macrophages infiltration, and macrophage polarization were examined by RT-qPCR, and immunohistochemistry. Results: We found that the transplantation of WAT derived from normal mice improved functional blood flow in HFD-fed mice compared to mice transplanted with BAT and sham-treated mice. WAT transplantation increased the recruitment of pericytes associated with nascent blood vessels, but did not affect capillary formation. Furthermore, transplantation of WAT ameliorated HFD-induced insulin resistance, M2 macrophage predominance and the release of arteriogenic factors in ischemic muscles. Mice receiving WAT also displayed a marked reduction in several proinflammatory cytokines. In contrast, mice transplanted with BAT were glucose intolerant and demonstrated increased IL-6 levels in ischemic muscles. Conclusion: These results indicate that transplantation of adipose tissue elicits improvements in blood perfusion and beneficial effects on systemic glucose homeostasis and could be a promising therapeutic option for the treatment of diabetic peripheral arterial disease. |
| Databáze: | OpenAIRE |
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