The effectiveness of eugenol against cisplatin-induced ototoxicity
| Autor: | Korhan Kilic, Serkan Yildirim, Muhammed Sedat Sakat, Gizem Eser, Fazile Nur Ekinci Akdemir, Ahmet Kiziltunc |
|---|---|
| Přispěvatelé: | Belirlenecek |
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
antioxidant medicine.medical_treatment animal cell 010501 environmental sciences Pharmacology medicine.disease_cause 01 natural sciences Antioxidants necrosis Rats Sprague-Dawley chemistry.chemical_compound 0302 clinical medicine 8-Hydroxy-2?-deoxyguanosine biochemical analysis Sprague dawley rats oxidative stress rat stria animal endothelium cell 8-Hydroxy-2′-deoxyguanosine glutathione peroxidase Saline antineoplastic agent connective tissue outer hair cell ganglion Sprague Dawley rat drug effect malonaldehyde single drug dose spiral ganglion staining lcsh:Otorhinolaryngology lcsh:RF1-547 superoxide dismutase enzyme activity Cochlea Eugenol ototoxicity 030220 oncology & carcinogenesis immunohistochemistry histopathology Female medicine.drug spontaneous otoacoustic emission animal experiment Otoacoustic Emissions Spontaneous Antineoplastic Agents Article animal tissue enzyme blood level 03 medical and health sciences Ototoxicity medicine Animals controlled study Hearing Loss 0105 earth and related environmental sciences Cisplatin distortion product otoacoustic emission nerve cell degeneration nonhuman business.industry disease model Corti organ hearing impairment medicine.disease DPOAE Rats drug efficacy signal noise ratio Disease Models Animal Otorhinolaryngology chemistry external auditory canal Oxidative stress DNA damage pathology hyperemia business Cisplatin-induced ototoxicity |
| Zdroj: | Brazilian Journal of Otorhinolaryngology v.85 n.6 2019 Brazilian Journal of Otorhinolaryngology Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial (ABORL-CCF) instacron:ABORL-CCF Brazilian Journal of Otorhinolaryngology, Vol 85, Iss 6, Pp 766-773 (2019) |
| ISSN: | 1808-8686 |
| Popis: | Introduction: Ototoxicity refers to cellular damage or function impairment developing in the inner ear in association with any therapeutic agent or chemical substance, and still represents the principal side-effect restricting the use of cisplatin. Objective: The aim of this study was to perform a biochemical, functional and histopathological investigation of the potential protective effect of eugenol against cisplatin-induced ototoxicity. Methods: The study was performed with 24 female Sprague Dawley rats. Distortion product otoacoustic emissions tests were performed on all animals, which were randomized into four equal groups. A single intraperitoneal dose of 15 mg/kg cisplatin was administered to cisplatin group, while the eugenol group received 100 mg/kg eugenol intraperitoneal for five consecutive days. 100 mg/kg eugenol was administered to cisplatin + eugenol group for 5 days. On the third day, these rats were received a single dose of 15 mg/kg cisplatin. The control group was given 8 mL/kg/day intraperitoneal saline solution for five days. The distortion product otoacoustic emissions test was repeated 24 h after the final drug administration. All animals were sacrificed, and the cochleas were subsequently used for biochemical and histopathological examinations. Results: Cisplatin caused oxidative stress in the cochlea, impaired the cochlear structure and significantly reduced signal noise ratio levels. Administration of eugenol together with cisplatin reversed these effects and provided functional, biochemical and histopathological protection. Conclusion: The study findings represent the first indication in the literature that eugenol may protect against ototoxicity by raising levels of antioxidant enzymes and lowering those of oxidant parameters. © 2018 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial This study was performed in accordance with the PHS Policy on Humane Care and Use of Laboratory Animals, the NIH Guide for the Care and Use of Laboratory Animals, and the Animal Welfare Act (7 U.S.C. et seq.); the animal use protocol was approved by the Institutional Animal Care and Use Committee (IACUC) of Ataturk University. |
| Databáze: | OpenAIRE |
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