Cell-specific expression of functional glucose transporter 8 in mammary gland
| Autor: | Lorena Mardones, Katia Muñoz, Marcelo Villagrán |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
GLUT8 Glucose uptake Mammary gland Glucose Transport Proteins Facilitative Biophysics Gene Expression Biochemistry Cell Line Mice 03 medical and health sciences Mammary Glands Animal 0302 clinical medicine Lysosome Lactation Adipocytes medicine Animals Humans Molecular Biology Mice Inbred BALB C biology Chemistry Myoepithelial cell Glucose transporter Colocalization Epithelial Cells Cell Biology Cell biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 567:125-130 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2021.06.037 |
| Popis: | Differentiated mammary epithelial cells are responsible for milk synthesis during lactation, supporting early postnatal life in mammals. These cells are found in the terminal alveoli of a secretory epithelium, which is surrounded by myoepithelial cells and a stroma rich in fatty tissue. The aim of this study was to explore the cell-specific expression of the glucose transporter GLUT8 in mammary gland and evaluate its functionality for glucose transport, in order to confirm its role in lactose synthesis. Our histological results revealed that GLUT8 is expressed in adipocytes and the epithelial and myoepithelial cells in mammary gland, with a predominant intracellular granular pattern. Colocalization studies of endogenous and green fluorescent protein fused GLUT8 revealed their expressions in lysosome and Golgi, respectively, with Pearson's coefficient correlations of 0.82 ± 0.05 and 0.68 ± 0.16. Functional studies of dileucine to dialanine mutant of GLUT8 showed a fructose-sensitive 2-deoxy glucose uptake at a rate of 83.3 pmoles/(min∗106 cells), 7 folds over empty vector, with a 60 ± 4 and 72 ± 6% decline in 2-deoxy glucose in the presence of 20 and 50 mM fructose, respectively. We concluded that functional GLUT8 is expressed in mammary gland, localizing in mammary epithelial and myoepithelial cells, and adipocytes. In lactation, GLUT8 is expressed mainly in luminal epithelial cells, at the compartments of the endomembrane system. It is necessary to explore the physiological/pathological functions of GLUT8 in mammary gland, including its role in lactation. |
| Databáze: | OpenAIRE |
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