Enhanced autophagy in Becn1(F121A/F121A) knockin mice counteracts aging-related neural stem cell exhaustion and dysfunction
| Autor: | Salwa Sebti, Álvaro F. Fernández, Chenran Wang, Michael Haas, Jun-Lin Guan, Syn Kok Yeo, Zhongju Zou, Beth Levine |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Aging Neurogenesis Subventricular zone Biology 03 medical and health sciences Mice Neuroblast Neural Stem Cells medicine Autophagy Animals Molecular Biology 030102 biochemistry & molecular biology Neurodegeneration Cell Biology BECN1 medicine.disease Neural stem cell Cell biology 030104 developmental biology medicine.anatomical_structure nervous system Beclin-1 Stem cell Research Paper |
| Zdroj: | Autophagy |
| Popis: | Macroautophagy/autophagy is emerging as a major pathway that regulates both aging and stem cell function. Previous studies have demonstrated a positive correlation of autophagy with longevity; however, these studies did not directly address the consequence of altered autophagy in stem cells during aging. In this study, we used Becn1(F121A/F121A) knockin mice (designated as Becn1 KI mice) with the F121A allele in the autophagy gene Becn1 to investigate the consequences of enhanced autophagy in postnatal neural stem cells (NSCs) during aging. We found that increased autophagy protected NSCs from exhaustion and promoted neurogenesis in old (≥18-months-old) mice compared with age-matched wild-type (WT) mice, although it did not affect NSCs in young (3-months-old) mice. After pharmacologically-induced elimination of proliferative cells in the subventricular zone (SVZ), there was enhanced re-activation of quiescent NSCs in old Becn1 KI mice as compared to those in WT mice, with more efficient exit from quiescent status to generate proliferative cells and neuroblasts. Moreover, there was also improved maintenance and increased neuronal differentiation of NSCs isolated from the SVZ of old Becn1 KI mice in in vitro assays. Lastly, the increased neurogenesis in Becn1 KI mice was associated with better olfactory function in aged animals. Together, our results suggest a protective role of increased autophagy in aging NSCs, which may help the development of novel strategies to treat age-related neurodegeneration. Abbreviations: ATG: autophagy related; Baf A(1): bafilomycin A(1); Becn1: beclin 1; BrdU: bromodeoxyuridine/5-bromo-2ʹ-deoxyuridine; DCX: doublecortin; GFAP: glial fibrillary acidic protein; GFP: green fluorescent protein; H&E: hematoxylin and eosin; HSCs: hematopoietic stem cells; KI: knockin; MAP1LC3B/LC3: microtubule associated protein 1 light chain 3 beta; mo: month; NSCs: neural stem cells; OB: olfactory bulb; RB1CC1: RB1-inducible coiled-coil 1; ROS: reactive oxygen species; SOX2: SRY (sex determining region Y)-box 2; SGZ: subgranular zone; SVZ: subventricular zone; TMZ: temozolomide; WT: wild type. |
| Databáze: | OpenAIRE |
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