Intrahepatic bacterial metataxonomic signature in non-alcoholic fatty liver disease
| Autor: | Martin Enrique Garaycoechea, Carlos José Pirola, Adrián Emanuel Salatino, Gustavo Osvaldo Castaño, Cinthia Belen Fijalkowky, Silvia Cristina Sookoian, Maria Silvina Landa |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
DNA Bacterial Male medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Microbial DNA LIVER BIOPSY Firmicutes Overweight Gastroenterology Non-alcoholic Fatty Liver Disease RNA Ribosomal 16S Internal medicine Proteobacteria Gammaproteobacteria medicine Humans Obesity biology medicine.diagnostic_test Bacteroidetes NONALCOHOLIC STEATOHEPATITIS business.industry Fatty liver Lachnospiraceae FATTY LIVER purl.org/becyt/ford/3.1 [https] Middle Aged Bioquímica y Biología Molecular medicine.disease biology.organism_classification Obesity Morbid Medicina Básica Phenotype Liver Case-Control Studies Liver biopsy purl.org/becyt/ford/3 [https] Female medicine.symptom INTESTINAL MICROBIOLOGY business |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1468-3288 0017-5749 |
| DOI: | 10.1136/gutjnl-2019-318811 |
| Popis: | Objective: We aimed to characterise the liver tissue bacterial metataxonomic signature in two independent cohorts of patients with biopsy-proven non-alcoholic fatty liver disease (NAFLD) diagnosis, as differences in the host phenotypic features - from moderate to severe obesity - may be associated with significant changes in the microbial DNA profile. Design and methods: Liver tissue samples from 116 individuals, comprising of 47 NAFLD overweight or moderately obese patients, 50 NAFLD morbidly obese patients elected for bariatric surgery and 19 controls, were analysed using high-throughput 16S rRNA gene sequencing. Results: Liver bacterial DNA profile significantly differs between morbidly obese and non-morbidly obese patients with NAFLD. Bacteroidetes (p=1.8e-18) and Firmicutes (p=0.0044) were over-represented in morbidly obese patients and Proteobacteria (p=5.2e-10) - specifically Gammaproteobacteria and Alphaproteobacteria, and Deinococcus-Thermus (p=0.00012) - were over-represented in the non-morbidly obese cohort. Cohort-specific analysis of liver microbial DNA signatures shows patterns linked to obesity. The imbalance in Proteobacteria (Alpha or Gamma) among non-morbidly obese patients, and Peptostreptococcaceae, Verrucomicrobia, Actinobacteria and Gamma Proteobacteria DNA among morbidly obese patients was associated with histological severity. Decreased amounts of bacterial DNA from the Lachnospiraceae family were associated with more severe histological features. Proteobacteria DNA was consistently associated with lobular and portal inflammation scores. Microbial DNA composition corresponded to predicted functional differences. Conclusion: This is the first comprehensive study showing that the liver tissue of NAFLD patients contains a diverse repertoire of bacterial DNA (up to 2.5×104 read counts). The liver metataxonomic signature may explain differences in the NAFLD pathogenic mechanisms as well as physiological functions of the host. Fil: Sookoian, Silvia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Salatino, Adrián Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Castaño, Gustavo Osvaldo. Gobierno de la Ciudad de Buenos Aires. Hospital "Dr. Abel Zubizarreta"; Argentina Fil: Landa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Fijalkowky, Cinthia Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Garaycoechea, Martin Enrique. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; Argentina Fil: Pirola, Carlos José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina |
| Databáze: | OpenAIRE |
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