Chromolaena laevigata (Asteraceae) as a source of endophytic non-aflatoxigenic Aspergillus flavus: chemical profile in different culture conditions and biological applications
| Autor: | Maria Helena Sarragiotto, Marta Regina Barrotto do Carmo, Darlon Irineu Bernardi, Debora Cristina Baldoqui, Érica Benassi Zanqueta, João Alencar Pamphile, Márcia Regina Pereira Cabral, Rodolfo Bento Balbinot, Benedito Prado Dias Filho, Jean Eduardo Meneguello, Andressa Domingos Polli, João Lúcio de Azevedo, Josiane A Monteiro de Oliveira, Rosilene Fressatti Cardoso, Julio Cesar Polonio, Tania U. Nakamura, Eliana Harue Endo |
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| Rok vydání: | 2021 |
| Předmět: |
Aflatoxin
Microorganism Aspergillus flavus Biology Microbiology Plant use of endophytic fungi in defense 03 medical and health sciences chemistry.chemical_compound Aflatoxins Microbial ecology Endophytes Media Technology Food microbiology Biotechnology and Industrial Microbiology - Research Paper Food science 030304 developmental biology Biological Products 0303 health sciences 030306 microbiology Chromolaena food and beverages biology.organism_classification Antimicrobial chemistry Kojic acid PLANTAS HOSPEDEIRAS |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Braz J Microbiol |
| Popis: | Endophytes are microorganisms that form symbiotic relationships with their host. These microorganisms can produce a variety of secondary metabolites, some of which have inhibitory effects on pests and pathogens or even act to promote plant growth. Due to these characteristics, these microorganisms are used as sources of biologically active substances for a wide range of biotechnological applications. Based on that, the aim of this study was to evaluate the production of metabolites of the endophytic Aspergillus flavus CL7 isolated from Chromolaena laevigata, in four different cultivation conditions, and to determine the antimicrobial, cytotoxic, antiviral, and antioxidant potential of these extracts. The multiphasic approach used to identify this strain was based on morphology and ITS gene sequence analysis. The chemical investigation of A. flavus using potato dextrose and minimal medium, using both stationary and agitated methods, resulted in the isolation of kojic acid, α-cyclopiazonic acid, and 20,25-dihydroxyaflavinine. Another 18 compounds in these extracts were identified by UHPLC-HRMS/MS, of which dideacetyl parasiticolide A has been described for the first time from A. flavus. Aflatoxins, important chemomarkers of A. flavus, were not detected in any of the extracts, thus indicating that the CL7 strain is non-aflatoxigenic. The biological potential of all extracts was evaluated, and the best results were observed for the extract obtained using minimal medium against Trichophyton rubrum and Mycobacterium tuberculosis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-021-00502-6. |
| Databáze: | OpenAIRE |
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