Exploiting ligand-protein conjugates to monitor ligand-receptor interactions

Autor: Kai Johnsson, Hirohito Haruki, Monica Rengifo Gonzalez
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Proteomics
lcsh:Medicine
Expression
Ligands
Biochemistry
01 natural sciences
Analytical Chemistry
Drug Discovery
lcsh:Science
0303 health sciences
Multidisciplinary
Cell fusion
Chemistry
Small molecule
Cell biology
Dihydrofolate-Reductase
Medicine
In-Vivo
Protein Binding
Research Article
Biotechnology
Drugs and Devices
Drug Research and Development
Recombinant Fusion Proteins
Cells
Fluorescence Polarization
010402 general chemistry
Protein–protein interaction
03 medical and health sciences
Throughput Screening Assays
Chemical Biology
Protein Interactions
Biology
030304 developmental biology
Ligand
lcsh:R
Proteins
Binding
Fusion protein
0104 chemical sciences
Förster resonance energy transfer
Microscopy
Fluorescence
Affinity
Small Molecules
Biophysics
lcsh:Q
Escherichia-Coli
Fluorescence anisotropy
Conjugate
Zdroj: PLoS ONE, Vol 7, Iss 5, p e37598 (2012)
PLoS ONE
ISSN: 1932-6203
Popis: We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins. © 2012 Haruki et al.
Databáze: OpenAIRE
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