Expression of murine STF-1, a putative insulin gene transcription factor, in β cells of pancreas, duodenal epithelium and pancreatic exocrine and endocrine progenitors during ontogeny
| Autor: | Marc Montminy, Christopher V.E. Wright, R. Stein, Laura W. Gamer, J. Leonard, Y. Guz, G. Teitelman |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Cell type Duodenum Gene Expression Biology Models Biological Epithelium Immunoenzyme Techniques Islets of Langerhans Mice Internal medicine medicine Animals Insulin Molecular Biology Homeodomain Proteins Pancreatic duct Pancreatic islets Cell biology Endocrinology medicine.anatomical_structure Pancreatic bud PAX4 Beta cell RFX6 Pancreas Developmental Biology |
| Zdroj: | Development. 121:11-18 |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | The XlHbox 8 homeodomain protein of Xenopus and STF-1, its mammalian homolog, are selectively expressed by β cells of adult mouse pancreatic islets, where they are likely to regulate insulin expression. We sought to determine whether the expression of the homeobox protein/s during mouse embryonic development was specific to β cells or, alternatively, whether XlHbox 8/STF-1 protein/s were initially expressed by multipotential precursors and only later became restricted to the insulin-containing cells. With two antibodies, we studied the localization of STF-1 during murine pancreatic development. In embryos, as in adults, STF-1 was expressed by most β cells, by subsets of the other islet cell types and by mucosal epithelial cells of the duodenum. In addition, most epithelial cells of the pancreatic duct and exocrine cells of the pancreas transiently contained STF-1. We conclude that in mouse, STF-1 not only labels a domain of intestinal epithelial cells but also provides a spatial and temporal marker of endodermal commitment to a pancreatic and subsequently, to an endocrine β cell fate. We propose a model of pancreatic cell development that suggests that exocrine and endocrine (α, β, ∂ and PP) cells arise from a common precursor pool of STF-1+ cells and that progression towards a defined mono-specific non-β cell type is correlated with loss of STF-1 expression. |
| Databáze: | OpenAIRE |
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