Bacillus amyloliquefaciens exopolysaccharide preparation induces glucagon-like peptide 1 secretion through the activation of bitter taste receptors
| Autor: | Hsueh-Ling Cheng, Wei-Wen Sung, Jia-Hong Chang, Marisa Zakiya Ulfa, Jing-Hong Tu, Jyun-Sian Yu |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_element
Enteroendocrine cell 02 engineering and technology Calcium Biochemistry Green fluorescent protein Cell Line Receptors G-Protein-Coupled 03 medical and health sciences Calcium imaging Structural Biology Bacillus amyloliquefaciens Glucagon-Like Peptide 1 Humans Secretion Receptors Somatostatin Receptor Molecular Biology 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug Somatostatin receptor HEK 293 cells Polysaccharides Bacterial General Medicine 021001 nanoscience & nanotechnology Cell biology HEK293 Cells chemistry Gene Expression Regulation 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 185 |
| ISSN: | 1879-0003 |
| Popis: | The exopolysaccharide preparation of Bacillus amyloliquefaciens amy-1 (EPS) regulates glycemic levels and promotes glucagon-like peptide 1 (GLP-1) secretion in vivo and in vitro. This study aimed to identify the molecular mechanism underlying EPS-induced GLP-1 secretion. HEK293T cells stably expressing human Gα-gustducin were used as a heterologous system for expressing the genes of human bitter taste receptor (T2R) 10, 14, 30, 38 (PAV), 38 (AVI), 43, and 46, which were expressed as recombinant proteins with an N-terminal tag composed of a Lucy peptide and a human somatostatin receptor subtype 3 fragment for membrane targeting and a C-terminal red fluorescent protein for expression monitoring. EPS induced a dose-dependent calcium response from the human NCI-H716 enteroendocrine cell line revealed by fluorescent calcium imaging, but inhibitors of the G protein-coupled receptor pathway suppressed the response. EPS activated heterologously expressed T2R14 and T2R38 (PAV). shRNAs of T2R14 effectively inhibited EPS-induced calcium response and GLP-1 secretion in NCI-H716 cells, suggesting the involvement of T2R14 in these effects. The involvement of T2R38 was not characterized because NCI-H716 cells express T2R38 (AVI). In conclusion, the activation of T2Rs mediates EPS-induced GLP-1 secretion from enteroendocrine cells, and T2R14 is a critical target activated by EPS in these cells. |
| Databáze: | OpenAIRE |
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