Molecular Detection of Helicobacter pylori and its Antimicrobial Resistance in Brazzaville, Congo

Autor:	Rachelle Moyen, Ange Antoine Abena, Francis Mégraud, Jamuna Vadivelu, Esther Nina Ontsira Ngoyi, Lucie Bénéjat, Khean-Lee Goh, Jean Maurille Ouamba, Roland Bienvenu Ossibi Ibara, Rock Fabien Niama, O. Obengui, Elodie Sifré, Jean Rosaire Ibara, Philippe Lehours, Armelle Ménard, Etienne Nguimbi, Blaise Irénée Atipo Ibara, Philestine Clausina Mikolele Ahoui Apendi, F. Yala
Rok vydání:	2015
Předmět:	Adult Male Adolescent medicine.drug_class Tetracycline Microbial Sensitivity Tests Real-Time Polymerase Chain Reaction Microbiology Helicobacter Infections Young Adult Antibiotic resistance Levofloxacin Clarithromycin Drug Resistance Multiple Bacterial Medicine Humans Aged biology Helicobacter pylori business.industry Gastroenterology General Medicine Middle Aged bacterial infections and mycoses Antimicrobial Quinolone biology.organism_classification Anti-Bacterial Agents Molecular Typing Infectious Diseases Real-time polymerase chain reaction Congo Female business medicine.drug Fluoroquinolones
Zdroj:	Helicobacter. 20(4)
ISSN:	1523-5378
Popis:	Back ground Helicobacter pylori infection is involved in several gastroduodenal diseases which can be cured by antimicrobial treatment. The aim of this study was to determine the prevalence of H. pylori infection and its bacterial resistance to clarithromycin, fluoroquinolones, and tetracycline in Brazzaville, Congo, by using molecular methods. Material and methods A cross- sectional study was carried out between September 2013 and April 2014. Biopsy specimens were obtained from patients scheduled for an upper gastrointestinal endoscopy and were sent to the French National Reference Center for Campylobacters and Helicobacters where they were tested by molecular methods for detection of H. pylori and clarithromycin resistance by real-time PCR using a fluorescence resonance energy transfer-melting curve analysis (FRET-MCA) protocol, for detection of tetracycline resistance by real-time PCR on 16S rRNA genes (rrnA and rrnB), for detection of point mutations in the quinolone resistance-determining regions (QRDR) of H. pylori gyrA gene, associated with resistance to quinolones, by PCR and sequencing. Results This study showed a high H. pylori prevalence (89%), low rates of clarithromycin and tetracycline resistance (1.7% and 2.5%, respectively), and a high rate of quinolone resistance (50%). Conclusion Therefore, the use of standard clarithromycin-based triple therapy is still possible as an empiric first-line treatment as well as prescription of bismuth-based quadruple therapy, which includes tetracycline, but not a levofloxacin-based triple therapy because of the high rate of resistance to fluoroquinolones.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ad76411769d59aca0ccff0cce8b36d8 https://pubmed.ncbi.nlm.nih.gov/25585658 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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