Vision-dependent specification of cell types and function in the developing cortex
Autor: | Sarah Cheng, Salwan Butrus, Liming Tan, Runzhe Xu, Srikant Sagireddy, Joshua T. Trachtenberg, Karthik Shekhar, S. Lawrence Zipursky |
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Rok vydání: | 2021 |
Předmět: |
Male
Neurons Vision Binocular Gene Expression Profiling Gene Expression Regulation Developmental Glutamic Acid Membrane Proteins Nerve Tissue Proteins General Biochemistry Genetics and Molecular Biology Article Mice Inbred C57BL Animals Newborn Animals RNA-Seq Transcriptome Biomarkers Vision Ocular gamma-Aminobutyric Acid Visual Cortex |
Zdroj: | Cell |
ISSN: | 1097-4172 |
Popis: | The role of postnatal experience in sculpting cortical circuitry, while long appreciated, is poorly understood at the level of cell types. We explore this in the mouse primary visual cortex (V1) using single-nucleus RNA sequencing, visual deprivation, genetics, and functional imaging. We find that vision selectively drives the specification of glutamatergic cell types in upper layers (L) (L2/3/4), while deeper-layer glutamatergic, GABAergic, and non-neuronal cell types are established prior to eye opening. L2/3 cell types form an experience-dependent spatial continuum defined by the graded expression of ∼200 genes, including regulators of cell adhesion and synapse formation. One of these genes, Igsf9b, a vision-dependent gene encoding an inhibitory synaptic cell adhesion molecule, is required for the normal development of binocular responses in L2/3. In summary, vision preferentially regulates the development of upper-layer glutamatergic cell types through the regulation of cell-type-specific gene expression programs. |
Databáze: | OpenAIRE |
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