MiR-511 mimic transfection inhibits the proliferation, invasion of osteosarcoma cells and reduces metastatic osteosarcoma tumor burden in nude mice via targeting MAPK1
| Autor: | Junxue Wu, Chao Zhang, Lu Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
Cancer Research proliferation Mitogen-activated protein Tumor burden Mice Nude Bone Neoplasms Mg63 cell MAPK1 Mice 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Genetics medicine Animals Humans Neoplasm Invasiveness neoplasms Cell Proliferation 030304 developmental biology Mitogen-Activated Protein Kinase 1 Osteosarcoma 0303 health sciences MiR-511 Lung business.industry General Medicine Transfection invasion medicine.disease Xenograft Model Antitumor Assays Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Metastatic osteosarcoma Cancer research business Research Article |
| Zdroj: | Cancer Biomarkers |
| ISSN: | 1875-8592 1574-0153 |
| DOI: | 10.3233/cbm-190534 |
| Popis: | Osteosarcoma, a highly aggressive cancer, can rapidly metastasize to distant organs such as lung, liver, brain. Despite much progress in the therapeutic regime has been made, the prognosis of osteosarcoma remains poor. In present study, microRNA-511 (miR-511) is lowly expressed in osteosarcoma cells, including MG63, U-2 OS, Saos-2 cells, while mitogen activated protein kinase1 (MAPK1) is highly expressed in osteosarcoma cells. Interestingly, MAPK1 might be a target of miR-511. We found that overexpression of miR-511 by miR-511 mimic transfection may result to low expression of MAPK1. Further study showed that miR-511 mimic inhibits the development of osteosarcoma MG63 cell, including proliferation and invasion. Moreover, miR-511 mimic transfection reduces metastatic osteosarcoma tumor burden in nude mice. These activities are mediated by targeting MAPK1. Our study provides a new sight for the molecular pathogenesis of osteosarcoma. |
| Databáze: | OpenAIRE |
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