In vitro antiviral activity of four isothiazole derivatives against poliovirus type 1
| Autor: | M.R. Pinizzotto, Francesco Guerrera, Adriana Garozzo, Furneri Pm, M.G. La Rosa, A. Castro, E. Geremia |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pharmacology
Isothiazole Poliovirus RNA Biological activity Biology Virus Replication medicine.disease_cause Antiviral Agents Uridine Thiazoles chemistry.chemical_compound Cytopathogenic Effect Viral Viral replication chemistry Biochemistry Cell culture Virology medicine Humans RNA Viral Mode of action Cells Cultured |
| Zdroj: | Antiviral Research. 19:29-41 |
| ISSN: | 0166-3542 |
| DOI: | 10.1016/0166-3542(92)90054-9 |
| Popis: | The in vitro effects of four isothiazoles [5,5′-diphenyl-3,3′-diisothiazole disulfide, 5-phenyl-3-mercapto-isothiazole, 5,5′-(4-chlorophenyl)-3,3′-diisothiazole disulfide, and 5-(4-chlorophenyl)-3-mercapto-isothiazole] on poliovirus type 1 were studied. The derivatives tested demonstrated remarkable viral inhibition, with a higher selectivity index than the previously studied iminodithiole precursors. Under one-step growth conditions, all the isothiazole derivatives caused the greatest activity if added during or after (within 1 h) poliovirus adsorption. These data suggest interference with early events of viral replication. [5-3H]Uridine incorporation into RNA showed that the compounds tested reduced poliovirus RNA synthesis, which was completely shut off after 2 h of incubation and reduced by 50–60% after 4 h. Also, pretreatment of the cell cultures with the compounds for 24 h caused a substantial inhibition of viral replication. The data suggest that the four isothiazole derivatives may have a multi-step antiviral mode of action different from their iminodithiole precursors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|