Vitamin D is Not Associated With Severity in NAFLD: Results of a Paired Clinical and Gene Expression Profile Analysis
| Autor: | Anna Mae Diehl, Dawn Piercy, Cynthia D. Guy, Yuval A. Patel, Cynthia A. Moylan, Manal F. Abdelmalek, Ricardo Henao |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pathology Vitamin D-binding protein 25-Hydroxyvitamin D3 1-alpha-hydroxylase Peroxisome Proliferator-Activated Receptors Parathyroid hormone Vitamin D3 24-Hydroxylase Gastroenterology Severity of Illness Index 0302 clinical medicine Non-alcoholic Fatty Liver Disease Cytochrome P-450 CYP3A Vitamin D Vitamin D-Binding Protein Middle Aged Liver Parathyroid Hormone Cholestanetriol 26-Monooxygenase 030211 gastroenterology & hepatology Female Adult medicine.medical_specialty Nuclear Receptor Coactivators macromolecular substances digestive system vitamin D deficiency Article 03 medical and health sciences Internal medicine Severity of illness medicine Vitamin D and neurology Humans RNA Messenger Cytochrome P450 Family 2 Retrospective Studies 25-Hydroxyvitamin D3 1-alpha-Hydroxylase Glycated Hemoglobin Hepatology business.industry Gene Expression Profiling nutritional and metabolic diseases medicine.disease Vitamin D Deficiency digestive system diseases Gene expression profiling 030104 developmental biology Cross-Sectional Studies Retinoid X Receptors nervous system Case-Control Studies Linear Models Receptors Calcitriol business |
| Popis: | The pathogenesis of nonalcoholic fatty liver disease (NAFLD) is complex. Vitamin D (VitD) has been implicated in NAFLD pathogenesis because it has roles in immune modulation, cell differentiation and proliferation, and regulation of inflammation. We evaluated the association of VitD levels, hepatic gene expression for VitD metabolizing genes, and NAFLD histological severity.Two adult cohorts, controls (n=39) and NAFLD (n=244), who underwent liver biopsy were compared. Two-sided t-tests or Wilcoxon's rank-sum tests for continuous predictors and chi-squared tests or Fisher's exact tests for categorical variables were used to analyze the association of VitD and NAFLD. Generalized linear models were used to analyze the association of VitD levels and VitD metabolizing genes with histological severity of NAFLD while adjusting for potential confounders and correcting for multiple comparisons.NAFLD patients were more likely than controls to have higher HbA1c (6.5±1.2 vs 5.9±1.0; P=0.009), a risk factor for VitD deficiency. However, no difference in VitD levels was observed between groups. VitD levels did not correlate with the severity of hepatic steatosis, lobular or portal inflammation, or ballooned hepatocytes after adjusting for confounding factors. Furthermore, no association was noted between VitD deficiency or differential expression of genes involved in VitD metabolism and severity of hepatic fibrosis or any other histologic feature of NAFLD.Neither VitD deficiency, nor hepatic expression of VitD-related genes, associate with the presence or histologic severity of NAFLD in patients. Hence, despite preclinical evidence implicating VitD in NAFLD pathogenesis, VitD deficiency does not appear to be associated with NAFLD severity in humans. |
| Databáze: | OpenAIRE |
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