Isolation and structure determination of new linear azole-containing peptides spongiicolazolicins A and B from Streptomyces sp. CWH03
| Autor: | Hideo Dohra, Hiroyuki Nakagawa, Hisayuki Komaki, Issara Kaweewan, Mana Suzuki, Masayuki Hayakawa, Hikaru Hemmi, Shinya Kodani, Hideki Yamamura |
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| Rok vydání: | 2020 |
| Předmět: |
Azoles
DNA Bacterial Stereochemistry Peptide Streptomyces spongiicola Linear azole-containing peptide Biosynthesis Applied Microbiology and Biotechnology Streptomyces 03 medical and health sciences Tandem Mass Spectrometry RNA Ribosomal 16S Gene cluster MS/MS Gene Peptide sequence Phylogeny 030304 developmental biology chemistry.chemical_classification 0303 health sciences Strain (chemistry) biology 030306 microbiology Fatty Acids Sequence Analysis DNA General Medicine biology.organism_classification NMR chemistry Peptides Two-dimensional nuclear magnetic resonance spectroscopy Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 105:93-104 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-020-11016-w |
| Popis: | Linear azole-containing peptides are a class of ribosomally synthesized and post-translationally modified peptides. We performed a chemical investigation on marine actinomycetes, and new linear azole-containing peptides named spongiicolazolicins A and B were found in the MeOH extracts of a newly isolated strain Streptomyces sp. CWH03 (NBRC 114659) and two strains of S. spongiicola (strain HNM0071T: DSM 103383T and strain 531S: NBRC 113560). The strain Streptomyces sp. CWH03 was indicated to be a new species closely related to S. spongiicola by phylogenetic analysis using the genome sequence. The new peptides named spongiicolazolicins A and B were isolated from the cell of Streptomyces sp. CWH03. The partial structure of spongiicolazolicin A was determined by 2D NMR experiments. Based on data of MS/MS experiments, the chemical structures of spongiicolazolicins A and B were proposed using the amino acid sequence deduced from the precursor-encoding gene, which was found from whole-genome sequence data of Streptomyces sp. CWH03. The biosynthetic gene cluster of spongiicolazolicins was proposed based on comparative analysis with that of a known linear azole peptide goadsporin. KEY POINTS: • Streptomyces sp. CWH03 was a new species isolated from marine sediment. • New linear azole-containing peptides named spongiicolazolicins A and B were isolated. • Biosynthetic pathway of spongiicolazolicins was proposed. |
| Databáze: | OpenAIRE |
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