Cardiomyocyte‐Specific Telomere Shortening is a Distinct Signature of Heart Failure in Humans
| Autor: | Ofer Harel, Foteini Mourkioti, Maryam Sharifi-Sanjani, Elisia D. Tichy, Kenneth B. Margulies, Kenneth C. Bedi, Nicholas M. Oyster |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty DNA damage Cardiomyopathy cardiomyocyte Disease 030204 cardiovascular system & hematology Ventricular Function Left 03 medical and health sciences 0302 clinical medicine Sex Factors Single-cell analysis Internal medicine medicine telomere length Humans Myocytes Cardiac Ventricular remodeling In Situ Hybridization Fluorescence Telomere Shortening Original Research Heart Failure Ventricular Remodeling business.industry Case-control study Age Factors Stroke Volume Hypertrophy Telomere medicine.disease Cell biology 030104 developmental biology Heart failure Case-Control Studies Cardiology Female Single-Cell Analysis Cardiology and Cardiovascular Medicine business Cardiomyopathies cardiomyopathy DNA Damage |
| Zdroj: | Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease |
| ISSN: | 2047-9980 |
| Popis: | Background Telomere defects are thought to play a role in cardiomyopathies, but the specific cell type affected by the disease in human hearts is not yet identified. The aim of this study was to systematically evaluate the cell type specificity of telomere shortening in patients with heart failure in relation to their cardiac disease, age, and sex. Methods and Results We studied cardiac tissues from patients with heart failure by utilizing telomere quantitative fluorescence in situ hybridization, a highly sensitive method with single‐cell resolution. In this study, total of 63 human left ventricular samples, including 37 diseased and 26 nonfailing donor hearts, were stained for telomeres in combination with cardiomyocyte‐ or α‐smooth muscle cell‐specific markers, cardiac troponin T, and smooth muscle actin, respectively, and assessed for telomere length. Patients with heart failure demonstrate shorter cardiomyocyte telomeres compared with nonfailing donors, which is specific only to cardiomyocytes within diseased human hearts and is associated with cardiomyocyte DNA damage. Our data further reveal that hypertrophic hearts with reduced ejection fraction exhibit the shortest telomeres. In contrast to other reported cell types, no difference in cardiomyocyte telomere length is evident with age. However, under the disease state, telomere attrition manifests in both young and older patients with cardiac hypertrophy. Finally, we demonstrate that cardiomyocyte‐telomere length is better sustained in women than men under diseased conditions. Conclusions This study provides the first evidence of cardiomyocyte‐specific telomere shortening in heart failure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text