Integration of genetically modified adult astrocytes into the lesioned rat spinal cord

Autor: Jacques Mallet, Philippe Pencalet, Alain Privat, Che Serguera, Olga Corti, Minerva Giménez y Ribotta
Přispěvatelé: Génétique moléculaire de la neurotransmission et des processus neurodégénératifs (LGMNPN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie et thérapie des déficits sensoriels et moteurs, Université Montpellier 2 - Sciences et Techniques (UM2)-IFR76-Institut National de la Santé et de la Recherche Médicale (INSERM), Association Française contre les Myopathies, Institut du Cerveau et de la Moelle Epinière (France)
Rok vydání: 2006
Předmět:
Pathology
Cell Transplantation
Green fluorescent protein
MESH: HIV-1
0302 clinical medicine
MESH: Glial Fibrillary Acidic Protein
MESH: Animals
Spinal cord injury
0303 health sciences
Glial fibrillary acidic protein
Gene Transfer Techniques
MESH: Fluorescent Dyes
Immunohistochemistry
MESH: Transplantation
Autologous
MESH: Cell Transplantation
surgical procedures
operative
medicine.anatomical_structure
Female
Astrocyte
medicine.medical_specialty
MESH: Rats
Green Fluorescent Proteins
MESH: Gene Transfer Techniques
Biology
Transplantation
Autologous
03 medical and health sciences
Cellular and Molecular Neuroscience
MESH: Green Fluorescent Proteins
Glial Fibrillary Acidic Protein
medicine
Animals
Vimentin
Autologous transplantation
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Spinal Cord Injuries
Spinal Cord Injuries
Fluorescent Dyes
030304 developmental biology
MESH: Rats
Inbred F344
MESH: Immunohistochemistry
medicine.disease
Spinal cord
Rats
Inbred F344
Rats
MESH: Astrocytes
Transplantation
Astrocytes
HIV-1
biology.protein
MESH: Vimentin
MESH: Female
Neuroscience
030217 neurology & neurosurgery
Ex vivo
Zdroj: Journal of Neuroscience Research
Journal of Neuroscience Research, Wiley, 2006, 83 (1), pp.61-7. ⟨10.1002/jnr.20697⟩
Journal of Neuroscience Research, 2006, 83 (1), pp.61-7. ⟨10.1002/jnr.20697⟩
ISSN: 1097-4547
0360-4012
DOI: 10.1002/jnr.20697
Popis: Combination of ex vivo gene transfer and cell transplantation is now considered as a potentially useful strategy for the treatment of spinal cord injury. In a perspective of clinical application, autologous transplantation could be an option of choice. We analyzed the fate of adult rat cortical astrocytes genetically engineered with a lentiviral vector transplanted into a lesioned rat spinal cord. Cultures of adult rat cortical astrocytes were infected with an HIV-1-derived vector (TRIP-CMV-GFP) and labeled with the fluorescent dye Hoechst. Transfected and labeled astrocyte suspension was injected at T11 in rats in which spinal cord transection at T7-T8 levels had been carried out 1 week earlier. Six weeks after grafting, the animals were sacrificed and transplants were retrieved either by Hoechst fluorescence or by immunohistochemistry for detection of glial fibrillary acidic protein (GFAP) and vimentin. Grafted astrocytes expressing green fluorescent protein (GFP) were found both at the injection and transection sites. Genetically modified astrocytes thus survived, integrated, and migrated within the host parenchyma when grafted into the completely transected rat spinal cord. In addition, they retained some ability to express the GFP transgene for at least 6 weeks after transplantation. Adult astrocytes infected with lentiviral vectors can therefore be a valuable tool for the delivery of therapeutic factors into the lesioned spinal cord.
Contract grant sponsor: Institut de Recherche sur la Moelle Epiniere;Contract grant sponsor: Association Francaise des Myopathies.
Databáze: OpenAIRE
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