| Autor: |
Herold, Rosanna, Scholtysik, Ren��, Moroniak, Selina, Weiss, Christel, Ishikawa, Hiroshi, Schroten, Horst, Schwerk, Christian |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.17125712.v1 |
| Popis: |
Additional file 1. Inhibition of the Erk1/2 and p38 signalling pathways by specific inhibitors. HIBCPP cells grown in the inverted culture system and infected for 4 h with the NmB strain MC58siaD- (Nm). Untreated cells were used as controls. The activation of Erk1/2 was inhibited by adding 25 ��M of the inhibitor U0126 (U) and detected using an immunoblot (A). The cells treated with PMA served as positive controls. Phosphorylation of p38 was inhibited by SB203580 (SB) at a concentration of 25 ��M. The functionality of the inhibitor was examined using an immunoblot of the p38 target protein MAPKAPK-2 (B). Treatment with anisomycin (Aniso) was used as positive control. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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