Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction
| Autor: | Hans Clevers, Dominique T. Brandt, Eduard Batlle, Leo S. Price, Johannes L. Bos, Jurgen Riedl |
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| Rok vydání: | 2005 |
| Předmět: |
rho GTP-Binding Proteins
Colon Receptor EphB2 Cell Down-Regulation Ephrin-B1 Biology Biochemistry Cell Movement Cell Line Tumor Precursor cell Cell Adhesion medicine Humans Ephrin Cell adhesion Cell Shape Molecular Biology digestive oral and skin physiology Erythropoietin-producing hepatocellular (Eph) receptor rap1 GTP-Binding Proteins Cell Biology Molecular biology Cell biology medicine.anatomical_structure Gene Expression Regulation ras GTPase-Activating Proteins Cell culture Rap1 Tyrosine kinase Research Article |
| Zdroj: | Biochemical Journal. 389:465-469 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj20050048 |
| Popis: | Ephrins are cell surface ligands that activate Eph receptor tyrosine kinases. This ligand–receptor interaction plays a central role in the sorting of cells. We have previously shown that the ephrinB–EphB signalling pathway is also involved in the migration of intestinal precursor cells along the crypts. Using the colon cell line DLD1 expressing the EphB2 receptor, we showed that stimulation of these cells with soluble ephrinB1 results in a rapid retraction of cell extensions and a detachment of cells. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. Importantly, when a constitutively active Rap1 mutant was introduced into these cells, ephrinB1-induced retraction was inhibited. From these results, we conclude that down-regulation of Rap1 is a prerequisite for ephrin-induced cell retraction in colon cells. |
| Databáze: | OpenAIRE |
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