The flavonoid baicalin counteracts ischemic and oxidative insults to retinal cells and lipid peroxidation to brain membranes

Autor: D. Ji, S. H. Jung, K. D. Kang, N. N. Osborne, T. A. Kamalden, R. J. Fawcett, R. Safa
Přispěvatelé: University of Groningen
Rok vydání: 2008
Předmět:
Retinal Ganglion Cells
genetic structures
Pharmacology
medicine.disease_cause
Culture Media
Serum-Free
Lipid peroxidation
chemistry.chemical_compound
OPTIC-NERVE HEAD
Ischemia
RADICAL PRODUCTION
Cells
Cultured
INDUCED APOPTOSIS
chemistry.chemical_classification
Chemistry
NEURONAL DEATH
Neuroprotection
medicine.anatomical_structure
Neuroprotective Agents
Biochemistry
Caspases
Reperfusion Injury
Hypoxia-Ischemia
Brain
OPEN-ANGLE GLAUCOMA
Injections
Intraperitoneal
Cell Survival
Retinal ganglion
Retina
Choline O-Acetyltransferase
Cellular and Molecular Neuroscience
HYDROGEN-PEROXIDE
medicine
Animals
SCUTELLARIA-BAICALENSIS-GEORGI
Baicalin
DIETARY FLAVONOIDS
Flavonoids
Reactive oxygen species
NITRIC-OXIDE
Retinal
Glaucoma
EPIGALLOCATECHIN GALLATE
Cell Biology
Hydrogen Peroxide
medicine.disease
eye diseases
Rats
Oxidative Stress
Flavonoid
Thy-1 Antigens
sense organs
Cell culture
Lipid Peroxidation
Reactive Oxygen Species
Oxidative stress
Photic Stimulation
Zdroj: Neurochemistry International, 53(6-8), 325-337. PERGAMON-ELSEVIER SCIENCE LTD
ISSN: 0197-0186
Popis: The purpose of the present Study was to determine whether the flavonoid, baicalin is effective at blunting the negative influence of ischemia/reperfusion to the rat retina ill situ and of various insults to a transformed retinal ganglion cells (RGC-5 cells) in culture.Baicalin was administered intraperitoneally just before and after all ischemic insult to retina of one eye of a rat. Ischemia was delivered by raising the intraocular pressure above the systolic blood pressure for 50 min. Seven days after ischemia, retinas were analysed for the localisation of various antigens. Retinal extracts were also analysed for various mRNAs. Moreover, the content of specific proteins was deduced in retinal and optic nerve extracts. Also, RGC-5 cells in culture were given one of three different insults, light (1000 1x for 2 days), hydrogen peroxide (200 mu M H(2)O(2) for 24 h) or serum deprivation (48 h) where cell Survival and reactive oxygen species (ROS) formation was assayed. Moreover, a lipid peroxidation assay was used to compare the antioxidant capacity of baicalin with the flavonoid, epigallocatechin gallate (EGCG).Ischemia/reperfusion to the retina affected the localisation of Thy-1 and choline acetyltransferase (ChAT) and the content of various proteins (optic nerve and retina) and mRNAs (retina). Importantly, baicalin Statistically blunted most Of the effects induced by ischemia/reperfusion. Only the increase ill caspase-8 and caspase-3 mRNAs caused by ischemia/reperfusion were unaffected by baicalin treatment. Baicalin also attenuated significantly the negative insult of light, hydrogen peroxide and serum withdrawal to RGC-5 cells. Ill the lipid peroxidation Studies, baicalin was also found to be equally effective as EGCG to act as all antioxidant. Significantly, the negative insult of serum withdrawal oil RGC-5 Cell Survival was blunted by baicalin but not by EGCG revealing the different properties of the two flavonoids. (C) 2008 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
Externí odkaz: