Rapid Detection and Characterization of Steroidal Saponins in the Root of Asparagus cochinchinensis by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

Autor: Renliang Jiang, Qing Zhang, Chuan Li, Yanlin Zhao, Yun Ling, Shuqin Yuan, Keyue Liu, Xin He, Qi Zhang, Yao Liang
Rok vydání: 2020
Předmět:
Zdroj: Journal of Chromatographic Science. 58:454-463
ISSN: 1945-239X
0021-9665
DOI: 10.1093/chromsci/bmaa006
Popis: The dried root of Asparagus cochinchinensis (RAC) has been used as an important traditional Chinese medicine for a long time in China. Steroidal saponins (SSs) are considered to be the main active ingredients of this herb. However, the isolation and structural determination of SSs from RAC are time-consuming and laborious. For this reason, the development of new methods for the separation and characterization of SSs is highly desirable. In this study, a new high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) method with precursor ions and the corresponding fragment ions was developed for the identification of SSs in RAC. Finally, 30 SSs have been detected and identified, including 17 potential new compounds. This is the first systematic study of SSs in RAC by HPLC-ESI-QTOF-MS/MS method.
Databáze: OpenAIRE
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