Ligand-gated chloride channel subunits encoded by the Haemonchus contortus and Ascaris suum orthologues of the Caenorhabditis elegans gbr-2 (avr-14) gene
| Autor: | Adrian J. Wolstenholme, Thomas M Skinner, Suchitra Jagannathan, Catherine L Critten, Lucy Horoszok, D L Laughton |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Nervous System Chloride Channels parasitic diseases Animals Tissue Distribution Amino Acid Sequence RNA Messenger Cloning Molecular Caenorhabditis elegans Molecular Biology Ascaris suum Gene Genes Helminth Sequence Homology Amino Acid biology Alternative splicing DNA biology.organism_classification Molecular biology Onchocerca volvulus Alternative Splicing Nematode Chloride channel Haemonchus Parasitology Ion Channel Gating Haemonchus contortus |
| Zdroj: | Molecular and Biochemical Parasitology. 103:129-140 |
| ISSN: | 0166-6851 |
| Popis: | The alternatively-spliced Caenorhabditis elegans gbr-2/avr-14 gene encodes two subunits of the nematode ligand-gated chloride channel family which forms an important molecular target for the avermectin and related anthelminthics. We used reverse transcriptase–polymerase chain reaction (RT–PCR) techniques to isolate cDNAs encoding the products of the gbr-2/avr-14 orthologues from the parasitic nematodes Haemonchus contortus and Ascaris suum. The predicted polypeptides possess all the characteristics of subunits of the ligand-gated chloride channels, sharing greater than 80% amino-acid identity with their counterparts in C. elegans and with partial sequences from the filarial species Onchocerca volvulus and Dirofilaria immitis. The pattern of alternative splicing of the gbr-2/avr-14 gene observed in C. elegans is conserved in H. contortus but may not be in A. suum. Affinity-purified anti-GBR-2 antibodies were used to study the expression of these subunits in adult worms and they reacted specifically with the nerve ring, the ventral and dorsal nerve cords, the anterior portion of the dorsal sub-lateral cord and motor-neuron commissures in H. contortus. Specific immunofluorescence of the nerve cords was confirmed in A. suum ; isolated muscle cells did not react with the antibody. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect