Serine–Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Scaffolding Protein CAST1/ERC2

Autor: Juan A. Godoy, Craig C. Garner, Viviana I. Torres, Eckart D. Gundelfinger, Carolina Montenegro-Venegas, Pedro Zamorano, Duxan Arancibia, Nibaldo C. Inestrosa, Matias Lira, Yocelin Cruz, Daniela P Barrera
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Scaffold protein
Gene isoform
genetics [Protein Serine-Threonine Kinases]
SRPK
coiled-coil
active zone
self-aggregation
CAST1/ERC2
synapse
Protein Serine-Threonine Kinases
metabolism [Cytoskeletal Proteins]
Article
Rats
Sprague-Dawley
Serine
Synapse
metabolism [Adaptor Proteins
Signal Transducing]
ddc:570
Animals
Humans
Active zone
Protein kinase A
lcsh:QH301-705.5
Cells
Cultured
chemistry [Synapses]
Adaptor Proteins
Signal Transducing
Neurons
Chemistry
HEK 293 cells
chemistry [Adaptor Proteins
Signal Transducing]
physiology [Protein Serine-Threonine Kinases]
metabolism [Synapses]
General Medicine
genetics [Protein Multimerization]
Embryo
Mammalian
Rats
Cell biology
genetics [Synapses]
Cytoskeletal Proteins
HEK293 Cells
lcsh:Biology (General)
metabolism [Neurons]
cytology [Neurons]
Synapses
chemistry [Cytoskeletal Proteins]
Female
Protein Multimerization
Drosophila Protein
Zdroj: Cells
Volume 8
Issue 11
Cells, Vol 8, Iss 11, p 1333 (2019)
Cells, 8(11):1333
Cells 8(11), 1333 (2019). doi:10.3390/cells8111333
ISSN: 2073-4409
DOI: 10.3390/cells8111333
Popis: Neurons release neurotransmitters at a specialized region of the presynaptic membrane, the active zone (AZ), where a complex meshwork of proteins organizes the release apparatus. The formation of this proteinaceous cytomatrix at the AZ (CAZ) depends on precise homo- and hetero-oligomerizations of distinct CAZ proteins. The CAZ protein CAST1/ERC2 contains four coiled-coil (CC) domains that interact with other CAZ proteins, but also promote self-assembly, which is an essential step for its integration during AZ formation. The self-assembly and synaptic recruitment of the Drosophila protein Bruchpilot (BRP), a partial homolog of CAST1/ERC2, is modulated by the serine-arginine protein kinase (SRPK79D). Here, we demonstrate that overexpression of the vertebrate SRPK2 regulates the self-assembly of CAST1/ERC2 in HEK293T, SH-SY5Y and HT-22 cells and the CC1 and CC4 domains are involved in this process. Moreover, the isoform SRPK2 forms a complex with CAST1/ERC2 when co-expressed in HEK293T and SH-SY5Y cells. More importantly, SRPK2 is present in brain synaptic fractions and synapses, suggesting that this protein kinase might control the level of self-aggregation of CAST1/ERC2 in synapses, and thereby modulate presynaptic assembly.
Databáze: OpenAIRE
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