Dopaminergic Neurodegeneration in the Mouse Is Associated with Decrease of Viscoelasticity of Substantia Nigra Tissue

Autor: Tonia Munder, Juergen Braun, Charlotte Klein, Barbara Steiner, Susanne Mueller, Ingolf Sack, Kerstin Riek, Elisabeth G. Hain
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Pathology
Dopamine
lcsh:Medicine
600 Technik
Medizin
angewandte Wissenschaften::610 Medizin und Gesundheit

Hippocampus
Biochemistry
Physical Chemistry
030218 nuclear medicine & medical imaging
Midbrain
chemistry.chemical_compound
Mice
0302 clinical medicine
Animal Cells
Materials Physics
Medicine and Health Sciences
lcsh:Science
Neurons
Multidisciplinary
Microglia
Chemistry
Viscosity
MPTP
Physics
Neurodegeneration
Dopaminergic
Brain
Neurochemistry
Parkinson Disease
Animal Models
Substantia Nigra
medicine.anatomical_structure
1-Methyl-4-phenyl-1
2
3
6-tetrahydropyridine

Physical Sciences
Female
Anatomy
Cellular Types
Neurochemicals
Brainstem
medicine.drug
Research Article
medicine.medical_specialty
Histology
Materials Science
Substantia nigra
Mouse Models
Research and Analysis Methods
03 medical and health sciences
Model Organisms
medicine
Animals
Macrophages
lcsh:R
Biology and Life Sciences
Cell Biology
medicine.disease
Elasticity
Magnetic resonance elastography
Mice
Inbred C57BL

Chemical Properties
nervous system
Cellular Neuroscience
lcsh:Q
Dopaminergics
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS ONE, Vol 11, Iss 8, p e0161179 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: The biomechanical properties of brain tissue are altered by histopathological changes due to neurodegenerative diseases like Parkinson's disease (PD). Such alterations can be measured by magnetic resonance elastography (MRE) as a non- invasive technique to determine viscoelastic parameters of the brain. Until now, the correlation between histopathological mechanisms and observed alterations in tissue viscoelasticity in neurodegenerative diseases is still not completely understood. Thus, the objective of this study was to evaluate (1) the validity of MRE to detect viscoelastic changes in small and specific brain regions: the substantia nigra (SN), midbrain and hippocampus in a mouse model of PD, and (2) if the induced dopaminergic neurodegeneration and inflammation in the SN is reflected by local changes in viscoelasticity. Therefore, MRE measurements of the SN, midbrain and hippocampus were performed in adult female mice before and at five time points after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin hydrochloride (MPTP) treatment specifically lesioning dopaminergic neurons in the SN. At each time point, additional mice were utilized for histological analysis of the SN. After treatment cessation, we observed opposed viscoelastic changes in the midbrain, hippocampus and SN with the midbrain showing a gradual rise and the hippocampus a distinct transient increase of viscous and elastic parameters, while viscosity and–to a lesser extent—elasticity in the SN decreased over time. The decrease in viscosity and elasticity in the SN was paralleled by a reduced number of neurons due to the MPTP-induced neurodegeneration. In conclusion, MRE is highly sensitive to detect local viscoelastic changes in specific and even small brain regions. Moreover, we confirmed that neuronal cells likely constitute the backbone of the adult brain mainly accounting for its viscoelasticity. Therefore, MRE could be established as a new potential instrument for clinical evaluation and diagnostics of neurodegenerative diseases.
Databáze: OpenAIRE