Dopaminergic Neurodegeneration in the Mouse Is Associated with Decrease of Viscoelasticity of Substantia Nigra Tissue
Autor: | Tonia Munder, Juergen Braun, Charlotte Klein, Barbara Steiner, Susanne Mueller, Ingolf Sack, Kerstin Riek, Elisabeth G. Hain |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
Pathology
Dopamine lcsh:Medicine 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit Hippocampus Biochemistry Physical Chemistry 030218 nuclear medicine & medical imaging Midbrain chemistry.chemical_compound Mice 0302 clinical medicine Animal Cells Materials Physics Medicine and Health Sciences lcsh:Science Neurons Multidisciplinary Microglia Chemistry Viscosity MPTP Physics Neurodegeneration Dopaminergic Brain Neurochemistry Parkinson Disease Animal Models Substantia Nigra medicine.anatomical_structure 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine Physical Sciences Female Anatomy Cellular Types Neurochemicals Brainstem medicine.drug Research Article medicine.medical_specialty Histology Materials Science Substantia nigra Mouse Models Research and Analysis Methods 03 medical and health sciences Model Organisms medicine Animals Macrophages lcsh:R Biology and Life Sciences Cell Biology medicine.disease Elasticity Magnetic resonance elastography Mice Inbred C57BL Chemical Properties nervous system Cellular Neuroscience lcsh:Q Dopaminergics 030217 neurology & neurosurgery Neuroscience |
Zdroj: | PLoS ONE, Vol 11, Iss 8, p e0161179 (2016) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | The biomechanical properties of brain tissue are altered by histopathological changes due to neurodegenerative diseases like Parkinson's disease (PD). Such alterations can be measured by magnetic resonance elastography (MRE) as a non- invasive technique to determine viscoelastic parameters of the brain. Until now, the correlation between histopathological mechanisms and observed alterations in tissue viscoelasticity in neurodegenerative diseases is still not completely understood. Thus, the objective of this study was to evaluate (1) the validity of MRE to detect viscoelastic changes in small and specific brain regions: the substantia nigra (SN), midbrain and hippocampus in a mouse model of PD, and (2) if the induced dopaminergic neurodegeneration and inflammation in the SN is reflected by local changes in viscoelasticity. Therefore, MRE measurements of the SN, midbrain and hippocampus were performed in adult female mice before and at five time points after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin hydrochloride (MPTP) treatment specifically lesioning dopaminergic neurons in the SN. At each time point, additional mice were utilized for histological analysis of the SN. After treatment cessation, we observed opposed viscoelastic changes in the midbrain, hippocampus and SN with the midbrain showing a gradual rise and the hippocampus a distinct transient increase of viscous and elastic parameters, while viscosity and–to a lesser extent—elasticity in the SN decreased over time. The decrease in viscosity and elasticity in the SN was paralleled by a reduced number of neurons due to the MPTP-induced neurodegeneration. In conclusion, MRE is highly sensitive to detect local viscoelastic changes in specific and even small brain regions. Moreover, we confirmed that neuronal cells likely constitute the backbone of the adult brain mainly accounting for its viscoelasticity. Therefore, MRE could be established as a new potential instrument for clinical evaluation and diagnostics of neurodegenerative diseases. |
Databáze: | OpenAIRE |
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