3-(4-methoxyl)-1-(2-(4-coumarin)prop)-2-en-1-one inhibits the differentiation of Gaoyou duck embryonic osteoclasts in vitro
| Autor: | Yihui Wang, Xianfu Liu, Dequn Niu, Wei Xue, Zheng Gong, Chuang Li, Xuemin Sun, Xue Li, Baoding Tang, Honggang Dai, Yingji Mao, Xi-Shuai Tong, Huang Yinjiu, Yingxiao Fu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Embryo
Nonmammalian Phalloidin Osteoclasts In Vitro Techniques 03 medical and health sciences chemistry.chemical_compound Multinucleate Osteoclast Coumarins medicine Animals Viability assay 030304 developmental biology 0303 health sciences biology Chemistry 0402 animal and dairy science Acid phosphatase Cell Differentiation 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Molecular biology In vitro Resorption medicine.anatomical_structure Ducks RANKL biology.protein Animal Science and Zoology |
| Zdroj: | Poultry science. 98(4) |
| ISSN: | 1525-3171 |
| Popis: | This study determined the influence of 3-(4-methoxyl)-1-(2-(4-coumarin)prop)-2-en-1-one (MCPEO) on the differentiation of Gaoyou duck embryo osteoclasts cultured in vitro. Bone marrow mononuclear cells (BM-MNCs) were harvested from 23-day-old Gaoyou duck embryos and induced by receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) in the presence of MCPEO at different concentrations (i.e., 1, 5, 10, 20, and 40 μM). Cell viability measurement, tartrate-resistant acid phosphatase (TRAP) staining, resorption activity assay, and co-staining with Tetramethylrhodamine (TRITC)-conjugated phalloidin and Hoechst 33,258 were conducted. Results indicated that MCPEO influenced the cell viability of the M-CSF + RANKL-induced BM-MNCs in a concentration-dependent manner, reduced the formation of positive multinucleated cells, and restrained the resorption capability of osteoclasts. Microfilament and nuclear staining indicated that MCPEO restricted the differentiation of BM-MNCs into large multinucleated osteoclasts. In short, MCPEO can inhibit the differentiation of BM-MNCs into mature osteoclasts in duck embryos. Therefore, MCPEO is a promising agent for the treatment of poultry osteoporosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|