In vitro and in vivo safety evaluation of low molecular weight chitosans prepared by hydrolyzing crab shell chitosans with bamboo shoots chitosanase
| Autor: | Ya-Min Chang, Yu-Jing Lee, Jyun-Kai Jhan, Chen-Tien Chang, Junn-Wang Liao, Yun-Chin Chung |
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| Rok vydání: | 2014 |
| Předmět: |
Male
No-observed-adverse-effect level Glycoside Hydrolases Bambusa In Vitro Techniques Pharmacology Toxicology medicine.disease_cause Chromosome aberration Mice In vivo Crustacea medicine Animals Chitosanase Chitosan Mice Inbred ICR Chemistry Hydrolysis General Medicine Acute toxicity Molecular Weight Immunology Toxicity Micronucleus Plant Shoots Genotoxicity Food Science |
| Zdroj: | Food and Chemical Toxicology. 71:10-16 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2014.05.016 |
| Popis: | Our previous study demonstrated that the oral administration of low molecular weight chitosans (LMWC), prepared by hydrolyzing crab shell chitosans with bamboo shoots chitosanase in an appropriate dose, reduced aristolochic acid-induced renal lesions in mice. The objectives of this study were to evaluate the safety of LMWC using genetic and animal toxicity assays. Two assays for genotoxicity were performed: the chromosomal aberration of Chinese hamster ovary cells (CHO-K1 cells) ( in vitro ) and micronucleus assays in mice ( in vivo ). Acute oral toxicity and 28-day repeated feeding toxicity tests were performed via the oral gavage method in Sprague–Dawley (SD) rats. LMWC did not induce an increase in micronucleus ratios in vivo , and the chromosome aberration assay indicated that the LMWC was safe in terms of clastogenicity in doses up to 5.0 mg/ml. No acute lethal effect at a maximum tested dose of 5.0 g LMWC/kg body weight (bw) was observed in rats. The results of the 28-day study revealed no adverse effects on the body weight, feed consumption, hematology, blood biochemical parameters, organ weights or pathology. The no observed adverse effect level (NOAEL) of LMWC in rats was 1.0 g/kg bw for the subacute toxicity study. |
| Databáze: | OpenAIRE |
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