Hepatocyte steatosis is a cytopathic effect of hepatitis C virus genotype 3

Autor: Gilles Mentha, Pierre-Jean Malé, Emiliano Giostra, Karim Abid, Bettina Borisch, Francesco Negro, Antoine Hadengue, Jean-Pierre Zarski, Rafael Quadri, Laura Rubbia-Brandt, Laurent Spahr
Rok vydání: 2000
Předmět:
Liver Cirrhosis
Male
Hepacivirus
Adult
Aged
Amino Acid Sequence/genetics
Antiviral Agents/therapeutic use
Fatty Liver/virology
Female
Genotype
Hepacivirus/genetics
Hepatitis C
Chronic/complications
Hepatitis C
Chronic/immunology
Hepatitis C
Chronic/pathology
Hepatitis C
Chronic/virology
Humans
Immunocompetence
Interferon-alpha/therapeutic use
Liver Cirrhosis/drug therapy
Liver Cirrhosis/virology
Liver Transplantation
Middle Aged
Molecular Sequence Data
Nucleocapsid Proteins/genetics
RNA
Viral/genetics
ddc:616.07
medicine.disease_cause
Cytopathic effect
ddc:616
ddc:615
ddc:617
biology
Fatty liver
virus diseases
Nucleocapsid Proteins
RNA
Viral
Hepatitis C virus
Antiviral Agents
Virus
Flaviviridae
medicine
Amino Acid Sequence
ddc:613
Liver Cirrhosis/drug therapy/virology
Hepatitis C
Chronic/complications/immunology/pathology/virology
Hepatology
Interferon-alpha
Hepatitis C
Chronic
medicine.disease
biology.organism_classification
Virology
Fatty Liver
Immunology
Steatosis
Zdroj: Journal of Hepatology, Vol. 33, No 1 (2000) pp. 106-15
Journal of hepatology, vol. 33, no. 1, pp. 106-115
ISSN: 0168-8278
DOI: 10.1016/s0168-8278(00)80166-x
Popis: Background/Aims: Patients infected with the hepatitis C virus (HCV) often have liver steatosis, suggesting the possibility of a viral cytopathic effect. The aim of this study was to correlate the occurrence and severity of liver steatosis with HCV RNA type, level and sequence of the core-encoding region. Methods: We scored the liver steatosis in 101 HCV-infected individuals carefully selected to exclude other risk factors of a fatty liver. Results were compared with HCV RNA genotype and level in serum and liver. In selected patients, we assessed the effect of antiviral therapy on steatosis and the relationship between nucleocapsid sequence heterogeneity and fat infiltration. Results: Steatosis was found in 41 (40.6%) patients, irrespective of sex, age or route of infection. HCV genotype 3 was associated with higher steatosis scores than other genotypes. A significant correlation between steatosis score and titer of intrahepatic HCV RNA was found in patients infected with genotype 3, but not in those infected with genotype 1. In selected patients, response to alpha-interferon was associated with the disappearance of steatosis. Analysis of the nucleocapsid of 14 HCV isolates failed to identify a sequence specifically associated with the development of steatosis. Conclusions: We provide virological and clinical evidence that the steatosis of the liver is the morphological expression of a viral cytopathic effect in patients infected with HCV genotype 3. At variance with published evidence from experimental models, the HCV nucleocapsid protein does not seem to fully explain the lipid accumulation in these patients.
Databáze: OpenAIRE
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