Cre-expressing neurons in visual cortex of Ntsr1-Cre GN220 mice are corticothalamic and are depolarized by acetylcholine
| Autor: | Björn Granseth, Sarah H. Lindstrom, Gonzalo Sánchez, Sofie C. Sundberg |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Action Potentials Mice Transgenic Sensory system Cholinergic Agonists In Vitro Techniques Biology Statistics Nonparametric Mice 03 medical and health sciences 0302 clinical medicine Thalamus Muscarinic acetylcholine receptor medicine Animals Receptors Neurotensin Visual Cortex Neurons Integrases Glutamate Decarboxylase General Neuroscience Forkhead Transcription Factors FOXP2 Claustrum Acetylcholine Repressor Proteins Luminescent Proteins Electrophysiology 030104 developmental biology Visual cortex medicine.anatomical_structure Nicotinic agonist nervous system Neuroscience 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Comparative Neurology. 526:120-132 |
| ISSN: | 0021-9967 |
| DOI: | 10.1002/cne.24323 |
| Popis: | The Ntsr1-Cre GN220 mouse expresses Cre-recombinase in corticothalamic (CT) neurons in neocortical layer 6. It is not known if the other major types of pyramidal neurons in this layer also express this enzyme. By electrophysiological recordings in slices and histological analysis of the uptake of retrogradely transported beads we show that Cre-positive neurons are CT and not corticocortical or corticoclaustral types. Furthermore, we show that Ntsr1-Cre-positive cells are immuno-positive for the nuclear transcription factor Forkhead box protein P2 (FoxP2). We conclude that Cre-expression is limited to a specific type of pyramidal neuron: CT. However, it appears as not all CT neurons are Cre-expressing; there are indications that the penetrance of the gene is about 90%. We demonstrate the utility of assigning a specific identity to individual neurons by determining that the CT neurons are potently modulated by acetylcholine acting on both nicotinic and muscarinic acetylcholine receptors. These results corroborate the suggested function of these neurons in regulating the gain of thalamocortical transfer of sensory information depending on attentional demand and state of arousal. |
| Databáze: | OpenAIRE |
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